Recombination between genes located on nonhomologous chromosomes in Saccharomyces cerevisiae.
Recombination between genes located on nonhomologous chromosomes in Saccharomyces cerevisiae.
复制标题
位于酿酒酵母非同源染色体上的基因之间的重组。
DOI：
10.1093/genetics/101.3-4.369
复制
发表时间：
1982
期刊：
影响因子：
3.3
通讯作者：
Petes,TD
中科院分区：
文献类型：
--
作者：
Mikus,MD;Petes,TD
我们构建了含有leu2基因或ura3基因的两种不同突变等位基因的酿酒酵母菌株。这些重复基因位于非同源染色体上，其中2个eu3等位基因位于染色体V和XII上，2个eu2等位基因位于染色体III和XII上。通过Leu+或Ura+回复突变体的产生来检测基因的两个突变拷贝之间的遗传相互作用。自发和紫外线照射诱导的回复突变体进行了检查。通过遗传和物理分析，我们已经表明Leu+或Ura+回复突变体可以通过各种不同的遗传相互作用产生。最常见的遗传相互作用类型是信息从一个重复序列到另一个重复序列的非互惠传递。我们还检测到重复基因之间的相互重组，导致染色体易位。
We constructed strains ofSaccharomyces cerevisiaethat contained two different mutant alleles of either theleu2gene or theura3gene. These repeated genes were located on nonhomologous chromosomes; the twoura3-alleles were located on chromosomesVandXIIand the twoleu2-alleles were located on chromosomesIIIandXII. Genetic interactions between the two mutant copies of a gene were detected by the generation of either Leu+or Ura+revertants. Both spontaneous and ultraviolet irradiation-induced revertants were examined. By genetic and physical analysis, we have shown that Leu+or Ura+revertants can arise by a variety of different genetic interactions. The most common type of genetic interaction is the nonreciprocal transfer of information from one repeat to the other. We also detected reciprocal recombination between repeated genes, resulting in reciprocally translocated chromosomes.