Development of beta-carotene-loaded poly(lactic acid)/hydroxyapatite core-shell nanoparticles for osteoblast differentiation
Development of beta-carotene-loaded poly(lactic acid)/hydroxyapatite core-shell nanoparticles for osteoblast differentiation
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DOI：
10.1080/21870764.2022.2127262
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发表时间：
2022-09
影响因子：
2.3
通讯作者：
S. Lee;Y. Sugimoto;Katsuya Kato;T. Miyajima;M. Sakurai;F. Nagata
中科院分区：
文献类型：
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作者：
S. Lee;Y. Sugimoto;Katsuya Kato;T. Miyajima;M. Sakurai;F. Nagata
摘要β-胡萝卜素（BC）是蔬菜中的一种营养素，也是维生素A的前体。据报道，BC具有抗癌、抗衰老和抗氧化特性，并可预防心脏病。近年来，由于BC对成骨细胞分化的刺激作用而受到了极大的关注。聚（乳酸）/羟基磷灰石（PLA/HAp）核-壳纳米粒子已被报道具有250%的水不溶性物质的负载能力，使用无表面活性剂的乳化方法。本研究制备了载BC的PLA/HAp核壳纳米粒子，并对其成骨分化行为进行了评价。成功地将BC负载到直径约为30 nm的PLA/HAp核壳纳米颗粒中。BC/装载PLA/HAp核壳纳米颗粒通过上调I型胶原、骨桥蛋白和骨钙蛋白表达刺激成骨细胞分化。此外，这些成骨细胞的基因表达水平显著高于那些由PLA/HAp核壳纳米颗粒刺激的无BC和在分化培养基（含有抗坏血酸和β-甘油磷酸盐）中培养的成骨细胞。PLA/HAp核壳纳米粒子与成骨细胞结合良好，具有良好的细胞相容性。因此，通过纳米颗粒将BC有效地递送到成骨细胞。这些结果表明，BC负载的PLA/HAp核壳纳米颗粒可以促进骨形成。图形摘要
ABSTRACT Beta-carotene (BC) is a nutrient in vegetables and a precursor of vitamin A. BC has been reported to have anticarcinogenic, antiaging, and antioxidation properties and prevents heart diseases. Recently, BC has gained significant attention due to stimulating effect on osteoblast differentiation. Poly(lactic acid)/hydroxyapatite (PLA/HAp) core-shell nanoparticles have been reported earlier with a load capacity of 250% for water-insoluble substances, using a surfactant-free emulsification method. In this work, PLA/HAp core-shell nanoparticles loaded with BC were prepared, and osteoblast differentiation behavior was evaluated. BC was successfully loaded into PLA/HAp core-shell nanoparticles with diameters of approximately 30 nm. BC/loaded PLA/HAp core-shell nanoparticles stimulated osteoblast differentiation by upregulating collagen type I, osteopontin, and osteocalcin expression. In addition, the gene expression levels of these osteoblasts were significantly larger than those stimulated by PLA/HAp core-shell nanoparticles without BC and cultured in a differential medium (with ascorbic acid and β-glycerophosphate). PLA/HAp core-shell nanoparticles showed satisfactory cytocompatibility because they were attached to the osteoblasts. Consequently, BC was effectively delivered to osteoblasts by nanoparticles. These results suggested that BC-loaded PLA/HAp core-shell nanoparticles could enhance bone formation. Graphical Abstract