Technical Feasibility of a Murine Model of Sleeve Gastrectomy with Ileal Transposition.
Technical Feasibility of a Murine Model of Sleeve Gastrectomy with Ileal Transposition.
复制标题
回肠转位袖状胃切除术小鼠模型的技术可行性。
DOI：
10.1007/s11695-018-3555-7
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发表时间：
2019
期刊：
影响因子：
2.9
通讯作者：
Martin,KathleenA
中科院分区：
文献类型：
--
作者：
Ying,LeeD;Breuer,GregoryA;Hubbard,MatthewO;Nadzam,GeoffreyS;Hwa,John;Martin,KathleenA
背景在大鼠和猪模型中，回肠移位袖状胃切除术在促进体重减轻方面上级单纯袖状胃切除术。由于缺乏小鼠模型，这一程序阻碍了努力了解其疗效的分子生理学基础。这项研究表明，长期生存的袖状胃切除术与回肠转位在mice.Materials and MethodsIn这项研究的技术可行性，袖状胃切除术与回肠转位（SGIT），袖状胃切除术（SG），或假手术（SH）进行7- 8周龄的C57 Bl/6 J小鼠（n= 8）。为了评估长期存活率，将小鼠置于致肥胖饮食中并每周称重，持续10周。肠身份的转座段进行了评估与基因表达分析的十二指肠，空肠，回肠特异性激素使用定量polymerase chain reaction.ResultsOverall，SGIT更好地防止体重增加比SG或假手术（10周后的重量：SH 45.3 ± 1.0克，SG 41.25 ± 1.6克，SGIT 35.4 ± 0.8克）。肠的身份（胃泌素，胆囊收缩素，和肽YY）的三个标志物的基因表达模式分析表明，回肠身份的换位段保持10周translocation.ConclusionsWe首次证明了一个可重复的小鼠模型的袖状胃切除术回肠转位后。利用该模型的未来研究将扩大我们对后肠调节饱腹感的分子途径的理解。
BackgroundSleeve gastrectomy with ileal transposition has been shown to be superior to sleeve gastrectomy alone for promoting weight loss in rat and porcine models. The absence of a mouse model for this procedure has impeded efforts to understand the molecular physiology underlying its efficacy. This study demonstrates the long-term survivability of sleeve gastrectomy with ileal transposition in mice.Materials and MethodsIn this study of technical feasibility, a sleeve gastrectomy with ileal transposition (SGIT), sleeve gastrectomy (SG), or sham surgery (SH) was performed on 7- to 8-week-old C57Bl/6J mice (n= 8 for each). To evaluate long-term survivability, mice were placed on an obesogenic diet and weighed weekly for 10 weeks. The intestinal identity of the transposed segment was assessed with gene expression analysis of duodenal-, jejunal-, and ileal-specific hormones using quantitative polymerase chain reaction.ResultsOverall, SGIT better prevented weight gain than the SG or sham procedures (10-week post-operative weight: SH 45.3 ± 1.0 g, SG 41.25 ± 1.6 g, SGIT 35.4 ± 0.8 g). Gene expression pattern analysis of three markers of intestinal identity (gastrin, cholecystokinin, and peptide YY) suggests that the ileal identity of the transposed segment is maintained 10 weeks after transposition.ConclusionsWe demonstrate for the first time a reproducible mouse model of sleeve gastrectomy with ileal transposition. Future studies utilizing this model will expand our understanding of the molecular pathways through which the hindgut regulates satiety.