Simple microcomputer system for mapping tissue sections with the light microscope.
Simple microcomputer system for mapping tissue sections with the light microscope.
复制标题
用于用光学显微镜绘制组织切片图的简单微型计算机系统。
DOI：
10.1016/0165-0270(88)90155-0
复制
发表时间：
1988
影响因子：
3
通讯作者：
Vicario,DS
中科院分区：
文献类型：
--
作者：
Alvarez-Buylla,A;Vicario,DS
我们描述了一种利用绘图管、显微镜舞台编码器和微型计算机对组织切片进行二维标测的方法。绘图管查看图形监视器并将屏幕光标的图像和屏幕上的菜单叠加在样本图像上。因此，在通过显微镜直接观察标本的同时，可以在没有阶段移动的情况下绘制每个显微领域中每个地标的位置。鼠标用于数据输入和程序控制。然后，以这种方式映射的字段被组合成完整的地图，其中可以包括线条图以及多达20种地标类型。映射的所有地标的坐标值都被存储，并保持可访问以供编辑和分析。制作了高分辨率的地块。专门的功能包括颗粒计数、面积和周长计算以及预定义要绘制的区域的周长限制器。该系统使用广泛可用的通用硬件。许多迄今耗时的任务，如详细绘制细胞位置、免疫细胞化学染色区域、退化纤维、神经元连接或任何其他解剖特征，可以在以前涉及的时间和精力的一小部分完成。由于数据已经是数字形式，因此可以在保持最高分辨率并支持统计分析的同时实现这些劳动力节约。
We describe a method for two-dimensional mapping of tissue sections that makes use of a drawing tube, microscope stage encoders and a microcomputer. The drawing tube views the graphics monitor and superimposes the image of the screen cursor and on-screen menus on the specimen image. Thus, the position of every landmark in each microscopic field can be mapped without stage movement while directly viewing the specimen through the microscope. A mouse is used for data entry and program control. Fields mapped in this way are then assembled into a complete map, which can include line drawings as well as up to 20 landmark types. The coordinate values of all landmarks mapped are stored and remain accessible for editing and analysis. High resolution plots are produced. Specialized functions include grain counting, area and perimeter calculations as well as a perimeter limiter that predefines the area to be mapped. The system uses general purpose hardware that is widely available. Many hitherto time-consuming tasks, such as detailed mapping of cell positions, regions of immunocytochemical staining, degenerating fibers, neuronal connections or any other anatomical feature, can be done in a fraction of the time and effort previously involved. These labor savings can be realized while maintaining the highest resolution and enabling statistical analysis since the data are already in digital form.