Mitochondrial DNA and their nuclear copies in the parasitic wasp Pteromalus puparum: A comparative analysis in Chalcidoidea
Mitochondrial DNA and their nuclear copies in the parasitic wasp Pteromalus puparum: A comparative analysis in Chalcidoidea
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寄生蜂 Pteromalus puparum 中的线粒体 DNA 及其核拷贝：小蜂总科的比较分析
DOI：
10.1016/j.ijbiomac.2018.10.039
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发表时间：
2019-01-01
影响因子：
8.2
通讯作者：
Ye, Gongyin
中科院分区：
文献类型：
--
作者：
Yan, Zhichao;Fan, Qi;Ye, Gongyin
小蜂总科（Chalcidoidea）是一个物种丰富、种类繁多的昆虫类群，具有重要的生态和经济意义。在这里，我们报告了一个完整的线粒体基因组Chalcidoidea从Pteromalus蛹（金小蜂科）。在其3308 bp的控制区检测到8个串联重复序列和6个反向重复序列。这个长而复杂的控制区可以解释在一些小蜂中完整的线粒体基因组扩增和测序的失败。除了37个典型的线粒体基因外，在对照区的另一端检测到额外相同的异亮氨酸tRNA（trill）。最近的线粒体基因重复表明，在chalcidoids的基因排列正在进行中。现有的小蜂线粒体基因组之间的比较揭示了快速的基因顺序重排的整体和高蛋白质取代率在大多数小蜂类群。此外，我们还鉴定了24个线粒体核基因序列（NUMT），总计9989 bp，其中3617 bp来自线粒体编码区。蛹中NUMTs的丰度仅为其亲戚Nasonia vitripennis的十二分之一。基于系统发育分析，我们提供的证据表明，更快的核降解速率有助于减少NUMT的数量在P.puparum。总的来说，我们的研究表明，在小蜂总科的线粒体进化和NUMT积累相当大的变化率异常高。(C)2018爱思唯尔出版社
Chalcidoidea (chalcidoid wasps) are an abundant and megadiverse insect group with both ecological and economical importance. Here we report a complete mitochondrial genome in Chalcidoidea from Pteromalus puparum (Pteromalidae). Eight tandem repeats followed by 6 reversed repeats were detected in its 3308 bp control region. This long and complex control region may explain failures of amplifying and sequencing of complete mitochondrial genomes in some chalcidoids. In addition to 37 typical mitochondrial genes, an extra identical isoleucine tRNA (trill) was detected at the opposite end of the control region. This recent mitochondrial gene duplication indicates that gene arrangements in chalcidoids are ongoing. A comparison among available chalcidoid mitochondrial genomes reveals rapid gene order rearrangements overall and high protein substitution rates in most chalcidoid taxa. In addition, we identified 24 nuclear sequences of mitochondrial origin (NUMTs) in P. puparum, summing up to 9989 bp, with 3617 bp of these NUMTs originating from mitochondrial coding regions. NUMTs abundance in P. puparum is only one-twelfth of that in its relative, Nasonia vitripennis. Based on phylogenetic analysis, we provide evidence that a faster nuclear degradation rate contributes to the reduced NUMT numbers in P. puparum. Overall, our study shows unusually high rates of mitochondrial evolution and considerable variation in NUMT accumulation in Chalcidoidea. (C) 2018 Published by Elsevier B.V.