Localization of neurons expressing choline acetyltransferase, serotonin and/or FMRFamide in the central nervous system of the decapod shore crab Hemigrapsus sanguineus
Localization of neurons expressing choline acetyltransferase, serotonin and/or FMRFamide in the central nervous system of the decapod shore crab Hemigrapsus sanguineus
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DOI：
10.1007/s00441-020-03309-3
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发表时间：
2020-11-25
影响因子：
3.6
通讯作者：
Dyachuk, Vyacheslav
中科院分区：
文献类型：
--
作者：
Kotsyuba, Elena;Dyachuk, Vyacheslav
虽然现在已经确定甲壳类动物的神经元含有多种递质物质，但对表达和共表达的模式或这种共传递的功能影响知之甚少。应用免疫组织化学结合激光扫描共聚焦显微镜技术，研究了胆碱乙酰转移酶(ChAT)、5-羟色胺(5-HT)和神经肽H-Phe-Met-Arg-Phe-NH2(FMRFamide)在中华绒螯蟹中枢神经系统(CNS)中的分布和可能的共存。ChAT在大脑和腹神经索(VNC)的所有区域都有表达，包括大、中、小神经元。在大多数情况下，ChAT、FMRFamide和5-HT在不同的神经元中表达，但也有一些ChAT-与FMRFamide或5-HT-LIR在中小型细胞中共存，大多数神经元免疫染色较弱。在大脑中，这种双重免疫标记主要在前脑的神经元中观察到，特别是在很大程度上，在中脑的局部嗅觉中间神经元中观察到。VNC中对ChAT、FMRFamide和5-羟色胺的染色最强的神经元簇位于食道下神经节，在某些情况下是共存的。这种共定位似乎与功能有关，因为它存在于中枢神经系统的区域，其特征是多个传入投射和输出到参与各种生理和行为过程的各种功能相关的中心。进一步阐明这些神经元的功能意义以及甲壳类动物中广泛存在的共同传递过程，应该会提供令人着迷的新见解。
Although it is now established that neurons in crustacea contain multiple transmitter substances, little is know about patterns of expression and co-expression or about the functional effects of such co-transmission. The present study was designed to characterize the distributions and potential colocalization of choline acetyltransferase (ChAT), serotonin (5-HT) and neuropeptide H-Phe-Met-Arg-Phe-NH2 (FMRFamide) in the central nervous system (CNS) of the Asian shore crab, Hemigrapsus sanguineus using immunohistochemical analyses in combination with laser scanning confocal microscopy. ChAT was found to be expressed by small, medium-sized, and large neurons in all regions of the brain and ventral nerve cord (VNC). For the most part, ChAT, FMRFamide, and 5-HT are expressed in different neurons, although some colocalization of ChAT- with FMRFamide- or 5-HT-LIR is observed in small and medium-sized cells, mostly neurons that immunostain only weakly. In the brain, such double immunolabeling is observed primarily in neurons of the protocerebrum and, to a particularly great extent, in local olfactory interneurons of the deutocerebrum. The clusters of neurons in the VNC that stain most intensely for ChAT, FMRFamide, and 5-HT, with colocalization in some cases, are located in the subesophageal ganglia. This colocalization appears to be related to function, since it is present in regions of the CNS characterized by multiple afferent projections and outputs to a variety of functionally related centers involved in various physiological and behavioral processes. Further elucidation of the functional significance of these neurons and of the widespread process of co-transmission in the crustaceans should provide fascinating new insights.