Extension in Flow of a DNA Molecule Tethered at One End
Extension in Flow of a DNA Molecule Tethered at One End
复制标题
一端束缚的 DNA 分子的流动延伸
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
B. Zimm
中科院分区：
文献类型：
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作者：
B. Zimm
一个理论被发展为蠕虫状链的稳态延伸，如DNA，在流动的液体中，分子的一端被拴在一个固定的物体上。这与Perkins，Smith，Larson和Chu最近对单独成像的DNA分子的实验有关。该理论是基于熟悉的奥森张量（也称为斯托克斯莱特）形式主义的链分子流体力学适用于珠段模型。在稳态条件下，不需要简正波分析。计算进行到几乎完全拉伸状态，在高流速，并重现实验以及与持久长度为49.3 nm的染色的DNA。通过本文所述的方法分析的流动中束缚的单分子的延伸测量似乎是确定单个分子持续长度的一种很好的方法。
A theory was developed for the steady-state extension of a wormlike chain, such as DNA, in flowing liquid with one end of the molecule tethered to a fixed object. This relates to recent experiments with individually imaged DNA molecules by Perkins, Smith, Larson, and Chu. The theory is based on the familiar Oseen tensor (also called Stokeslet) formalism of chain-molecule hydrodynamics applied to a bead-segment model. With the steady-state condition a normal-mode analysis is not necessary. The calculations are carried almost to the completely stretched state at high flow rates, and reproduce the experiments well with a persistence length of 49.3 nm for the stained DNA. Measurements of extension of tethered single molecules in flow analyzed by the method described here seem to make an excellent method for determining the persistence length of individual molecules.