Quantitative analysis of bone and soft tissue by micro-computed tomography: applications to ex vivo and in vivo studies.
Quantitative analysis of bone and soft tissue by micro-computed tomography: applications to ex vivo and in vivo studies.
复制标题
DOI：
10.1038/bonekey.2014.59
复制
发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Sophocleous, Antonia
中科院分区：
文献类型：
--
作者：
Campbell, Graeme M;Sophocleous, Antonia
微型计算机断层扫描 (micro-CT) 是一种高分辨率成像方式，能够分析体素大小约为 10μm 的骨骼结构。随着体内微型 CT 的发展，可以在一段时间内监测活体动物的疾病进展和治疗，这种方式已成为疾病进展和治疗期间骨结构临床前评估的标准工具。为了对显微 CT 研究进行有意义的比较，必须使用相同的数据采集参数和分析方法。本协议概述了目前在微 CT 研究中用于样品制备、扫描、重建和分析的常用程序。涵盖了股骨、胫骨、椎骨和小型啮齿类动物的整个新生儿身体的小梁骨和皮质骨的扫描和分析方法。讨论了使用 ScancoMedical 和 Bruker 提供的软件进行的分析程序，并概述了常规使用的骨结构参数。该协议还提供了专门用于体内扫描和分析的部分，其中涵盖麻醉、辐射剂量和图像配准的主题。由于使用微型 CT 来研究其他骨骼部位以及软组织的研究不断扩大，我们还对当前检查颅骨和下颌骨、脂肪组织、脉管系统、肿瘤严重程度和软骨的技术进行了回顾。包含推荐的进一步阅读和文献参考列表，以便为读者提供有关所描述方法的更多详细信息。
Micro-computed tomography (micro-CT) is a high-resolution imaging modality that is capable of analysing bone structure with a voxel size on the order of 10mum. With the development of in vivo micro-CT, where disease progression and treatment can be monitored in a living animal over a period of time, this modality has become a standard tool for preclinical assessment of bone architecture during disease progression and treatment. For meaningful comparison between micro-CT studies, it is essential that the same parameters for data acquisition and analysis methods be used. This protocol outlines the common procedures that are currently used for sample preparation, scanning, reconstruction and analysis in micro-CT studies. Scan and analysis methods for trabecular and cortical bone are covered for the femur, tibia, vertebra and the full neonate body of small rodents. The analysis procedures using the software provided by ScancoMedical and Bruker are discussed, and the routinely used bone architectural parameters are outlined. This protocol also provides a section dedicated to in vivo scanning and analysis, which covers the topics of anaesthesia, radiation dose and image registration. Because of the expanding research using micro-CT to study other skeletal sites, as well as soft tissues, we also provide a review of current techniques to examine the skull and mandible, adipose tissue, vasculature, tumour severity and cartilage. Lists of recommended further reading and literature references are included to provide the reader with more detail on the methods described.