Rapid confocal imaging of large areas of excised tissue with strip mosaicing
Rapid confocal imaging of large areas of excised tissue with strip mosaicing
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DOI：
10.1117/1.3582335
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发表时间：
2011-05-01
影响因子：
3.5
通讯作者：
Rajadhyaksha, Milind
中科院分区：
文献类型：
--
作者：
Abeytunge, Sanjee;Li, Yongbiao;Rajadhyaksha, Milind
快速和高分辨率的大面积组织成像可以在床边实现快速病理。高分辨率显微镜的有限视场要求将连续拍摄的多幅图像合并以覆盖大片区域。图像的这种合并或镶嵌需要很长的采集和处理时间，并且会产生伪像。为了减少时间和伪影，我们开发了一种在共聚焦显微镜上拼接的方法，以亚细胞细节对大范围切除组织的形态进行成像。通过获取长宽比为10：1或更高的图像条(而不是类似于1：1的标准)并在软件中对它们进行拼接，我们的方法在大约3分钟内对10x10 mm(2)的组织进行成像。这种方法，我们称之为“条状马赛克”，目前的速度是我们以前方法的三倍。(C)2011年，光学仪器工程师学会。[DOI：10.1117/1.3582335]
Imaging large areas of tissue rapidly and with high resolution may enable rapid pathology at the bedside. The limited field of view of high-resolution microscopes requires the merging of multiple images that are taken sequentially to cover a large area. This merging or mosaicing of images requires long acquisition and processing times, and produces artifacts. To reduce both time and artifacts, we developed a mosaicing method on a confocal microscope that images morphology in large areas of excised tissue with sub-cellular detail. By acquiring image strips with aspect ratios of 10:1 and higher (instead of the standard similar to 1:1) and "stitching" them in software, our method images 10x10 mm(2) area of tissue in about 3 min. This method, which we call "strip mosaicing," is currently three times as fast as our previous method. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3582335]