Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, mouse basigin, and chicken HT7 molecule.
Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, mouse basigin, and chicken HT7 molecule.
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DOI：
10.4049/jimmunol.149.3.847
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发表时间：
1992-08
影响因子：
4.4
通讯作者：
W. Kasinrerk;E. Fiebiger;I. Štefanov́a;T. Baumruker;W. Knapp;H. Stockinger
中科院分区：
文献类型：
--
作者：
W. Kasinrerk;E. Fiebiger;I. Štefanov́a;T. Baumruker;W. Knapp;H. Stockinger
最近发现，类风湿性关节炎和反应性关节炎患者的外周血粒细胞表达一种新定义的抗原，称为M6，与健康受试者的粒细胞相比，表达水平更高。我们在这里介绍了M6抗原的分子特征，并表明它是一种新的人类白细胞激活相关细胞表面糖蛋白。外周淋巴细胞不显著表达M6抗原，但在PHA激活的T细胞中3天即可表达。在构成表达M6抗原的单核细胞上，在粒细胞-巨噬细胞脑脊液刺激的第1天下调，但在第3天又被重新诱导。M6免疫沉淀物的SDS-PAGE分析显示，在非还原条件下有一条54 kDa的单一条带，在还原条件下移动到65 kDa。内切糖苷酶F对M6免疫沉淀物的处理表明，M6分子的50%是由N-连接的碳水化合物组成的。通过改进COS细胞克隆策略，我们获得了编码M6抗原的cDNA克隆。在Northern杂交中，M6基因与一条约1.7kb的mRNA转录本杂交。M6基因序列的比较分析表明，M6抗原是免疫球蛋白超家族的成员，与大鼠OX-47抗原、小鼠basigin(GP42)和鸡HT7分子同源。高度保守的跨膜结构域表明，M6Ag可能是质膜上多链复合体的一种成分。
Peripheral granulocytes from rheumatoid arthritis and reactive arthritis patients were recently found to express higher levels of a newly defined Ag, termed M6, in comparison to granulocytes from healthy subjects. We present here the molecular characterization of M6 Ag and show that it is a novel human leukocyte activation-associated cell surface glycoprotein. Peripheral lymphocytes do not significantly express M6 Ag, however, it appears upon 3-day PHA-activated T blasts. On monocytes, which constitutively express M6 Ag, it is down-regulated on day 1 but re-induced on day 3 of granulocyte-macrophage CSF stimulation. SDS-PAGE analysis of M6 immunoprecipitates shows a single band of 54 kDa under nonreducing conditions that shifts to 65 kDa under reducing conditions. Endoglycosidase F treatment of M6 immunoprecipitate reveals that 50% of the M6 molecule is composed of N-linked carbohydrates. By modifying the COS cell cloning strategy, we have isolated cDNA clones encoding M6 Ag. M6 cDNA hybridizes with a single mRNA transcript of approximately 1.7 kb in Northern blotting. Comparison analysis of the M6 sequence indicates that M6 Ag is a member of the Ig superfamily and the species homologue of rat OX-47 Ag, mouse basigin (gp42), and chicken HT7 molecule. The highly conserved remarkable transmembrane domain suggests that the M6 Ag may be a component of a multichain complex in the plasma membrane.