Evolution of multiple target-site resistance mechanisms in individual plants of glyphosate-resistant Eleusine indica from China.
Evolution of multiple target-site resistance mechanisms in individual plants of glyphosate-resistant Eleusine indica from China.
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DOI：
10.1002/ps.6527
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发表时间：
2021-06
影响因子：
4.1
通讯作者：
Chun Zhang;Chao Yu;Q. Yu;Wenlei Guo;Tai-Jie Zhang;X. Tian
中科院分区：
文献类型：
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作者：
Chun Zhang;Chao Yu;Q. Yu;Wenlei Guo;Tai-Jie Zhang;X. Tian
草甘膦在中国南方的各种情况下用于杂草控制已有四十年，并且大多数牛筋草种群被怀疑已经进化出对草甘膦的抗性。本研究调查了潜在的靶点草甘膦抗性机制，在六个现场收集，推定的草甘膦抗性（R）E。indica populations.结果6个R E.籼稻种群被证实相对于敏感（S）种群对草甘膦具有低（1.8至2.6倍）至中等（5.6至8.4倍）抗性。六十七草甘膦幸存的植物从六个R种群被用来研究靶点抗性机制。94%的转基因植株发生了5-烯醇式莽草酸3-磷酸合酶（EPSPS）基因过量表达（OE）和基因拷贝数变异（CNV），其中16%的转基因植株发生了P106 A突变，49%的转基因植株发生了T102 I + P106 S杂合突变（+P381 L）。此外，少量的R植株（6%）仅具有同源TIPS（加上P381 L）突变。EPSPS 5 '-UTR中的（CT）6插入突变总是与EPSPS OE和CNV相关。具有EPSPS OE/CNV（和P106 A）的后代植物显示出低水平（高达4.5倍）的草甘膦抗性。相比之下，TIPS突变纯合的植物表现出更高的草甘膦抗性（25倍），其次是该突变杂合的植物加上EPSPS OE/CNV（12倍）。结论E.华南地区籼稻群体普遍存在EPSPS OE/诱导/CNV，赋予低水平抗性。由于进化优势，获得TIPS突变和EPSPS OE/CNV的个体植物是有利的。（CT）6插入突变在EPSPS CNV中的作用值得进一步研究。本文受版权保护。All rights reserved.
BACKGROUND Glyphosate has been used for weed control in South China in various situations for four decades, and most Eleusine indica populations are suspected to have evolved resistance to glyphosate. This research investigated underling target-site glyphosate resistance mechanisms in six field-collected, putative glyphosate-resistant (R) E. indica populations. RESULTS The six R E. indica populations were confirmed to be low (1.8 to 2.6-fold) to moderately (5.6- to 8.4-fold) resistant to glyphosate relative to the susceptible (S) population. Sixty-seven glyphosate-surviving plants from the six R populations were used to examine target-site resistance mechanisms. Target-site 5-enolpyruvylshikimate3-phosphate synthase (EPSPS) overexpression (OE) (plus further induction by glyphosate treatment) and gene copy number variation (CNV) occurred in 94% R plants, and among them, 16% had the P106A mutation and 49% had the heterozygous double TIPS (T102I+P106S) mutation (plus P381L). In addition, a low number of R plants (6%) only had the homologous TIPS (plus P381L) mutation. The (CT)6 insertion mutation in the EPSPS 5'-UTR always associates with EPSPS OE and CNV. Progeny plants possessing EPSPS OE/CNV (and P106A) displayed low level (up to 4.5-fold) glyphosate resistance. In contrast, plants homozygous for the TIPS mutation displayed higher (25-fold) resistance to glyphosate and followed by plants heterozygous for this mutation plus EPSPS OE/CNV (12-fold). CONCLUSIONS Target-site glyphosate resistance in E. indica populations from South China is common with prevalence of EPSPS OE/induction/CNV conferring low level resistance. Individual plants acquiring both the TIPS mutation and EPSPS OE/CNV are favored due to evolutionary advantages. The role of (CT)6 insertion mutation in EPSPS CNV is worth further investigation. This article is protected by copyright. All rights reserved.