Viability of infective larvae of Haemonchus contortus, Ostertagia ostertagi, and Trichostrongylus colubriformis following exsheathment by various techniques
Viability of infective larvae of Haemonchus contortus, Ostertagia ostertagi, and Trichostrongylus colubriformis following exsheathment by various techniques
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DOI：
10.2307/3284123
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发表时间：
1996-02-01
影响因子：
1.3
通讯作者：
Johnson, SS
中科院分区：
文献类型：
--
作者：
Conder, GA;Johnson, SS
本研究探讨了反刍动物3种重要寄生虫（控制血矛线虫、奥斯特线虫和蛇形毛圆线虫）感染性幼虫的最佳离体条件。在重复实验中，将每种寄生虫的10（5）-10（6）个感染性幼虫（1-2月龄）的等分试样在4种脱鞘培养基（蒸馏水、Earle平衡盐溶液+二氧化碳、线虫洗涤缓冲液+二氧化碳或次氯酸钠）中孵育1或18小时。在每种情况下，测定幼虫脱鞘的百分比和对沙鼠的感染性。这些研究的结果表明，没有一个单一的exsheathing技术的研究是最佳的每一个寄生虫。此外，在使用脱鞘幼虫的体外研究中得出结论时必须谨慎，因为常规提供高脱鞘百分比的技术似乎也会降低生存力。
Various techniques were examined to determine optimum conditions for exsheathing infective larvae of 3 important ruminant parasites (Haemonchus controtus, Ostertagia ostertagi, and Trichostrongylus colubriformis). In repeated experiments, aliquots of 10(5)-10(6) infective larvae, 1-2 mo old, of each parasite were incubated in each of 4 exsheathing media (distilled water, Earle's balanced salt solution + carbon dioxide, nematode washing buffer + carbon dioxide, or sodium hypochlorite) for 1 or 18 hr. In each case, the percentage of larvae exsheathed and infectivity for jirds was determined. Results of these studies indicate that no single exsheathing technique of those studied is optimum for every parasite. In addition, caution must be used in drawing conclusions from in vitro studies using exsheathed larvae because techniques that routinely provide high percentages of exsheathment also appear to reduce viability.