Sound vibration, a non-invasive stress: antagonism by diazepam.
Sound vibration, a non-invasive stress: antagonism by diazepam.
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声音振动，一种非侵入性应激：地西泮的拮抗作用。
DOI：
10.1007/bf02244654
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发表时间：
1993
影响因子：
3.4
通讯作者：
Eisenberg,RM
中科院分区：
文献类型：
--
作者：
Eisenberg,RM
受到声音振动应激的大鼠血浆皮质酮（CS）突然增加。这种刺激是使用伯吉斯品牌的“振动雕刻机”可靠地产生的，这是一种用于雕刻的标准工具。将工具设置为“8”或粗调，将工具筒放置在动物的侧面，并将尖端抵住金属笼的侧面 15 秒。 15 分钟时血浆 CS 增加至 29.3±4.7 µg/dl，30 分钟时增加至 15.7±1.8 µg/dl。这些水平显着高于刺激前 2 小时用地西泮 5 mg/kg IV 预处理的动物（分别为 9.2±2.0 和 7.4±1.5 µg/dl）。在地西泮之前 30 分钟用 CGS-8216（苯二氮卓受体的混合激动剂/拮抗剂）2 mg/kg IV 预处理的动物，地西泮的保护作用被消除。单独给予 CGS-8216 的动物中，声音/振动使血浆 CS 显着升高；但是，这一升高明显低于媒介物处理的对照组。这种相对较低的血浆 CS 水平表明 CGS-8216 具有部分激动剂、地西泮样作用。实验是在具有慢性静脉导管的清醒、不受约束的雄性斯普拉格-道利大鼠中进行的。除了 15 秒的刺激暴露外，在连续采血期间，所有动物均保持隔离在声音衰减的单向视觉盒中。控制刺激暴露涉及类似的处理，无需打开雕刻工具。这些结果证明：1）该工具在提供可重复的压力刺激方面的有用性； 2）地西泮消除应激反应的能力； 3) CGS-8216可以拮抗地西泮的作用； 4) CGS-8216 部分激动剂作用的演示。
Rats, subjected to sound-vibration stress, showed an abrupt increase in plasma corticosterone (CS). This stimulation was reliably produced using a Burgess brand “vibro-graver,” a standard tool used for engraving. With the tool set at “8” or coarse, the barrel of the tool was placed on the animal's flank and the point held against the side of the metal cage for 15 s. Plasma CS increased to 29.3±4.7 µg/dl at 15 min and 15.7±1.8 µg/dl at 30 min. These levels were significantly higher than animals pretreated with diazepam, 5 mg/kg IV, 2 h prior to stimulation (9.2±2.0 and 7.4±1.5 µg/dl, respectively). Animals which were pretreated with CGS-8216 (a mixed agonist/antagonist at the benzodiazepine receptor), 2 mg/kg IV, 30 min prior to diazepam had the protective effects of diazepam abolished. Sound/vibration produced a significant elevation in plasma CS in animals given CGS-8216 alone; but, this elevation was significantly lower than in vehicle-treated controls. This comparatively lower plasma CS level suggests a partial-agonist, diazepam-like effect by CGS-8216. Experiments were done in conscious unrestrained male Sprague-Dawley rats with chronic IV catheters. Except for 15 s stimulation exposure, all animals remained isolated in sound-attenuated one-way vision boxes for the duration of the serial blood sampling. Control stimulation exposure involved similar handling without turning on the engraving tool. These results demonstrate: 1) the usefulness of this tool to provide a repeatable stress stimulus; 2) the ability of diazepam to abolish the stress response; 3) that CGS-8216 can antagonize the action of diazepam; and 4) a demonstration of the partial agonist effects of CGS-8216.