Dispersal of Carbapenemase blaVIM-1 Gene Associated with Different Tn402 Variants, Mercury Transposons, and Conjugative Plasmids in Enterobacteriaceae and Pseudomonas aeruginosa
Dispersal of Carbapenemase blaVIM-1 Gene Associated with Different Tn402 Variants, Mercury Transposons, and Conjugative Plasmids in Enterobacteriaceae and Pseudomonas aeruginosa
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DOI：
10.1128/aac.00783-09
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发表时间：
2010-01-01
影响因子：
4.9
通讯作者：
Canton, Rafael
中科院分区：
文献类型：
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作者：
Tato, Marta;Coque, Teresa M.;Canton, Rafael
报告了在金属β-内酰胺酶生产者流行率较低的地区，来自不同肠杆菌科和铜绿假单胞菌分离株的四种不同遗传平台中出现bla（Vim-1）。研究了2005 - 2007年回收的43株产Vim-1的分离株（包括19株阴沟肠杆菌、2株大肠埃希菌和2株铜绿假单胞菌分离株、18株肺炎克雷伯菌分离株和2株产酸克雷伯菌分离株），对应于15种脉冲场凝胶电泳类型。肠杆菌科分离株对应于医院疫情爆发，铜绿假单胞菌分离株零星回收。携带bla（Vim-1）的整合子（任意命名为A、B、C和D型）的遗传背景通过基于已知Tn 402和汞转座子的PCR作图和进一步测序来表征。在肠杆菌科分离株中，bla（Vim-1）是整合子的一部分，位于连接到Tn 21的In 2-Tn 402元件中，（A型; In 110-bla（Vim-1）-aacA 4-aadA 1）或在缺乏整个tni模块的Tn 402转座子中[B型; In 113-bla（Vim-1）-aacA 4-dhfr II（也称为dfrB 1）-aadA 1-catB 2]，转座子分别与IncHI 2或IncI 1质粒结合。在铜绿假单胞菌分离株中，bla（Vim-1）是位于携带tniB Delta 3和tniA（C型; bla（VIM-1）-aadA 1）或tniC和Delta tniQ（D型; bla（Vim-1）-aadB）的缺陷型Tn 402转座子中的新基因盒阵列的一部分，并且两种Tn 402变体都与30 kb的接合质粒相关。bla（Vim-1）的传播与不同的遗传结构和细菌宿主，描绘了一个复杂的出现和进化网络的情况下，在我们的设施，拉蒙卡哈尔大学医院，马德里，西班牙。了解bla（Vim-1）的复杂流行病学对于控制这种新出现的威胁是必要的。
The emergence of bla(VIM-1) within four different genetic platforms from distinct Enterobacteriaceae and Pseudomonas aeruginosa isolates in an area with a low prevalence of metallo-beta-lactamase producers is reported. Forty-three VIM-1-producing isolates (including 19 Enterobacter cloacae, 2 Escherichia coli, and 2 P. aeruginosa isolates, 18 Klebsiella pneumoniae isolate, and 2 Klebsiella oxytoca isolate) recovered from 2005 to 2007 and corresponding to 15 pulsed-field gel electrophoresis types were studied. The Enterobacteriaceae isolates corresponded to a hospital outbreak, and the P. aeruginosa isolates were sporadically recovered. The genetic context of the integrons carrying bla(VIM-1) (arbitrarily designated types A, B, C, and D) was characterized by PCR mapping based on known Tn402 and mercury transposons and further sequencing. Among Enterobacteriaceae isolates, bla(VIM-1) was part of integrons located either in an In2-Tn402 element linked to Tn21 (type A; In110-bla(VIM-1)-aacA4-aadA1) or in a Tn402 transposon lacking the whole tni module [type B; In113-bla(VIM-1)-aacA4-dhfrII (also called dfrB1)-aadA1-catB2] and the transposon was associated with an IncHI2 or IncI1 plasmid, respectively. Among P. aeruginosa isolates, bla(VIM-1) was part of a new gene cassette array located in a defective Tn402 transposon carrying either tniB Delta 3 and tniA (type C; bla(VIM-1)-aadA1) or tniC and Delta tniQ (type D; bla(VIM-1)-aadB), and both Tn402 variants were associated with conjugative plasmids of 30 kb. The dissemination of bla(VIM-1) was associated with different genetic structures and bacterial hosts, depicting a complex emergence and evolutionary network scenario in our facility, Ramon y Cajal University Hospital, Madrid, Spain. Knowledge of the complex epidemiology of bla(VIM-1) is necessary to control this emerging threat.