SEQUENCE, TISSUE DISTRIBUTION, AND CHROMOSOMAL LOCALIZATION OF MESSENGER-RNA ENCODING A HUMAN GLUCOSE TRANSPORTER-LIKE PROTEIN
SEQUENCE, TISSUE DISTRIBUTION, AND CHROMOSOMAL LOCALIZATION OF MESSENGER-RNA ENCODING A HUMAN GLUCOSE TRANSPORTER-LIKE PROTEIN
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DOI：
10.1073/pnas.85.15.5434
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发表时间：
1988-08-01
影响因子：
11.1
通讯作者：
BELL, GI
中科院分区：
文献类型：
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作者：
FUKUMOTO, H;SEINO, S;BELL, GI
通过与人HepG 2/红细胞葡萄糖转运蛋白cDNA的交叉杂交，已从成人肝和肾cDNA文库中分离出编码葡萄糖转运蛋白样蛋白的cDNA克隆。这个524个氨基酸的葡萄糖转运蛋白样蛋白的序列分析表明，它有55.5%的同一性HepG 2/红细胞葡萄糖转运蛋白，以及类似的结构组织。对成人组织中编码这种葡萄糖转运蛋白样蛋白的mRNA的组织分布的研究表明，肝脏中含量最高，肾脏和小肠中含量较低。在其他组织中，包括结肠、胃、大脑、骨骼肌和脂肪组织中的葡萄糖转运蛋白样mRNA的量低于我们测定的灵敏度水平。编码这种葡萄糖转运蛋白样蛋白的单拷贝基因已定位于q26.1 →。3号染色体q26.3区域。
cDNA clones encoding a glucose transporter-like protein have been isolated from adult human liver and kidney cDNA libraries by cross-hybridization with the human HepG2/erythrocyte glucose transporter cDNA. Analysis of the sequence of this 524-amino acid glucose transporter-like protein indicates that it has 55.5% identity with the HepG2/erythrocyte glucose transporter as well as a similar structural organization. Studies of the tissue distribution of the mRNA coding for this glucose transporter-like protein in adult human tissues indicate that the highest amounts are present in liver with lower amounts in kidney and small intestine. The amounts of glucose transporter-like mRNA in other tissues, including colon, stomach, cerebrum, skeletal muscle, and adipose tissue, were below the level of sensitivity of our assay. The single-copy gene encoding this glucose transporter-like protein has been localized to the q26.1 .fwdarw. q26.3 region of chromosome 3.