Characterization of the Arabidopsis lysine-rich arabinogalactan-protein AtAGP17 mutant (rat1) that results in a decreased efficiency of agrobacterium transformation
Characterization of the Arabidopsis lysine-rich arabinogalactan-protein AtAGP17 mutant (rat1) that results in a decreased efficiency of agrobacterium transformation
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DOI：
10.1104/pp.104.045542
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发表时间：
2004-08-01
期刊：
影响因子：
7.4
通讯作者：
Bacic, A
中科院分区：
文献类型：
--
作者：
Gaspar, YM;Nam, J;Bacic, A
阿拉伯半乳聚糖蛋白（AGPs）是一类广泛存在于植物中的复合蛋白聚糖。拟南芥rat 1突变体，以前的特点是耐根癌农杆菌根转化，是由于在富含赖氨酸的AGP，AtAGP 17的基因突变。我们发现，rat 1的表型与根中AGP 17的下调相关，这是由于T-DNA插入AGP 17的启动子。通过用野生型AGP 17基因或cDNA的花浸法补充Rat 1植物可以在几个独立的转化体中将植物恢复为野生型表型。基于PR 1基因表达的变化和自由水杨酸水平的降低农杆菌感染后，我们建议的机制，AGP 17允许农杆菌迅速减少系统获得性抗性反应在感染过程中。
Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans widely distributed in plants. The Arabidopsis rat1 mutant, previously characterized as resistant to Agrobacterium tumefaciens root transformation, is due to a mutation in the gene for the Lys-rich AGP, AtAGP17. We show that the phenotype of rat1 correlates with down-regulation of AGP17 in the root as a result of a T-DNA insertion into the promoter of AGP17. Complementation of rat1 plants by a floral dip method with either the wild-type AGP17 gene or cDNA can restore the plant to a wild-type phenotype in several independent transformants. Based on changes in PR1 gene expression and a decrease in free salicylic acid levels upon Agrobacterium infection, we suggest mechanisms by which AGP17 allows Agrobacterium rapidly to reduce the systemic acquired resistance response during the infection process.