Elevated levels of β-D-glucan in bronchoalveolar lavage fluid in patients with farmer's lung in Miyazaki, Japan
Elevated levels of β-D-glucan in bronchoalveolar lavage fluid in patients with farmer's lung in Miyazaki, Japan
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DOI：
10.1159/000098406
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发表时间：
2008-01-01
期刊：
影响因子：
3.7
通讯作者：
Nakazato, Masamitsu
中科院分区：
文献类型：
--
作者：
Ashitani, Jun-ichi;Kyoraku, Yuka;Nakazato, Masamitsu
背景：农民可能经常在发霉的干草中接触到β - d -葡聚糖，因为饲料秸秆可以全年储存。目的：本研究的目的是阐明农民肺部呼吸道中β - d -葡聚糖的水平是否过高，以及β - d -葡聚糖是否参与了这种疾病的发病机制。方法：测定10例农民肺患者、4例夏季型超敏性肺炎患者和10例健康志愿者支气管肺泡灌洗液（BALF）中β - d -葡聚糖水平。采用酶联免疫吸附法（ELISA）检测BALF中白细胞介素(IL)-8和肿瘤坏死因子(TNF)- α的水平。我们研究了β - d -葡聚糖对体外小气道上皮细胞（SAECs）核因子(NF)- κ B活化和IL-8和tnf - α释放的影响。结果：与夏季型HP患者和健康志愿者相比，农民肺患者BALF中β - d -葡聚糖水平升高。此外，农民肺BALF中的IL-8水平高于夏季型HP，两组患者的tnf - α水平相同，但与健康志愿者相比有所升高。高而非低浓度的β - d -葡聚糖可诱导saec中nf - κ B的活化。体外添加β - d -葡聚糖后，SAEC培养的上清液中IL-8水平升高。结论：农民肺患者BALF中β - d -葡聚糖水平较高，其可能通过激活呼吸道上皮细胞NF-kappa B而促进细胞因子的表达和释放。版权所有(C) 2007 S. Karger AG，巴塞尔
Background: Farmers may be often exposed to beta-D-glucan in moldy hay, since straw for feed can be stored throughout the year. Objectives: The aim of the present study was to clarify whether levels of beta-D-glucan, which modifies immune responses, are high in the respiratory tract in farmer's lung and whether beta-D-glucan participates in the pathogenesis of this condition. Methods: We measured beta-D-glucan levels in the bronchoalveolar lavage fluid (BALF) of 10 patients with farmer's lung, 4 with summer-type hypersensitivity pneumonitis (HP) and 10 healthy volunteers. Interleukin (IL)-8 and tumor necrosis factor (TNF)-alpha levels in BALF were measured by enzyme-linked immunosorbent assay (ELISA). We investigated the effects of beta-D-glucan on nuclear factor (NF)-kappa B activation and on the release of IL-8 and TNF-alpha from small airway epithelial cells (SAECs) in vitro. Results: beta-D-Glucan levels in the BALF of farmer's lung patients were increased compared to those in patients with summer-type HP and in healthy volunteers. Additionally, IL-8 levels in BALF were higher in farmer's lung than in summer-type HP, and TNF-alpha levels were equal in the two patient groups but raised compared to those in healthy volunteers. High, but not low, concentrations of beta-D-glucan were found to induce NF-kappa B activation in SAECs. IL-8 levels in the supernatant obtained from SAEC cultures were increased following the addition of beta-D-glucan in vitro. Conclusion: BALF from farmer's lung patients showed high beta-D-glucan levels, which may enhance the expression and release of cytokines through NF-kappa B activation in respiratory epithelial cells. Copyright (C) 2007 S. Karger AG, Basel.