Effect of egg weight on composition, embryonic growth, and expression of amino acid transporter genes in yolk sac membranes and small intestines of the domestic pigeon (Columba livia)
Effect of egg weight on composition, embryonic growth, and expression of amino acid transporter genes in yolk sac membranes and small intestines of the domestic pigeon (Columba livia)
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蛋重对家鸽（Columba livia）卵黄囊膜和小肠组成、胚胎生长和氨基酸转运蛋白基因表达的影响
DOI：
10.3382/ps/pew044
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发表时间：
2016-06-01
期刊：
影响因子：
4.4
通讯作者：
Gao, C. Q.
中科院分区：
文献类型：
--
作者：
Chen, M. X.;Li, X. G.;Gao, C. Q.
本试验旨在研究蛋重对家鸽蛋成分、胚发育及卵黄囊膜和小肠氨基酸转运基因表达的影响。取240枚受精卵，按卵重分为轻（LE）和重（HE）两组。每组取20只蛋，测量其成分，称重后放入孵卵箱中。在胚胎第9、11、13、15天和孵化第1天（DOH），每组15只胚胎/只进行胚胎生长测定，并收集标本。蛋鸡的蛋黄和蛋白质量均高于蛋鸡（P < 0.01）。从E13到DOH，高仔鸡的无蛋黄胚体和卵黄囊重量均高于低仔鸡（P < 0.05）。在E13 ~ E15阶段，高配蛋鸡的卵黄囊膜重显著高于低配蛋鸡（P < 0.05）， DOH残余卵黄囊含量显著高于低配蛋鸡（P < 0.01）。在E11 ~ E13期间，HE组的蛋黄吸收显著高于LE组（P < 0.05）。此外，在E13时，HE组蛋黄囊膜CAT2和PepT1 mRNA的丰度高于LE组（P < 0.05）。与LE组比较，E13组EAAT2基因表达量高于LE组，而E13组EAAT3基因表达量低于LE组（P < 0.05）。综上所述，我们的研究结果表明，蛋重影响了蛋的组成、胚胎发育以及卵黄囊膜和小肠中氨基酸转运基因的表达。
The objective of this study was to investigate the effect of egg weight on the composition of the egg, the growth of the embryo, and the expression of amino acid transporter genes in the yolk sac membranes and small intestines of the domestic pigeon (Columba livia). A total of 240 fertilized eggs were collected and divided into two groups based on the weight of the eggs, light (LE) and heavy (HE). The composition of 20 eggs from each group was measured, and the remaining eggs were weighed and placed in an incubator. On embryonic days (E) 9, 11, 13, and 15 and day of hatch (DOH), 15 embryos/hatchlings from each group were measured for embryonic growth, and samples were collected. The HE had heavier yolk and albumen weights than the LE (P < 0.01). Compared with the LE, the HE had heavier yolk-free embryonic body and yolk sac weights from E13 to DOH (P < 0.05). Additionally, the HE had larger yolk sac membrane weights from E13 to E15 (P < 0.05) and had more residual yolk sac content on DOH than those of the LE (P < 0.01). The yolk absorption was greater for the HE than for the LE from E11 to E13 (P < 0.05). Furthermore, the abundance of CAT2 and PepT1 mRNA in the yolk sac membranes was greater in the HE than in the LE on E13 (P < 0.05). Compared with the LE, the gene expression of EAAT2 in the intestine on E13 was greater in the HE, whereas the expression of EAAT3 was lower in the HE (P < 0.05). Taken together, our results suggest that egg weight influenced the composition of the eggs, embryonic development, and expression of amino acid transporter genes in the yolk sac membranes and small intestines of pigeon embryos.