Subolesin/akirin orthologs from Ornithodoros spp. soft ticks: Cloning, RNAi gene silencing and protective effect of the recombinant proteins
Subolesin/akirin orthologs from Ornithodoros spp. soft ticks: Cloning, RNAi gene silencing and protective effect of the recombinant proteins
复制标题
DOI：
10.1016/j.vetpar.2011.10.032
复制
发表时间：
2012-04-30
影响因子：
2.6
通讯作者：
Perez-Sanchez, Ricardo
中科院分区：
文献类型：
--
作者：
Manzano-Roman, Raul;Diaz-Martin, Veronica;Perez-Sanchez, Ricardo
Subolesin/akirin是一种充分表征的保护性抗原，在载体物种中高度保守，因此可能用于开发广谱疫苗，以控制节肢动物感染，包括硬蜱、蚊子、白蛉和家禽红螨鸡皮刺螨。如果证明软蜱subolesin直系同源物是保守的并且也诱导保护性免疫应答，则该疫苗也可以靶向软蜱。然而，迄今为止，没有软蜱subolesin直系同源物已被充分表征，也没有测试作为重组抗原在疫苗接种试验。本工作的目的是克隆和鉴定两种重要媒介软蜱Ornithodoros erraticus和O. moubata，以评估通过RNAi沉默subolesin基因的效果，并测试重组抗原在疫苗接种试验中的保护价值。结果表明，软蜱和硬蜱的subolesins在核苷酸和氨基酸序列上均高度保守，分别与硬蜱的subolesins有69%和74%的同源性。此外，我们证明了这两种软蜱都具有完全可操作的RNAi机制，并且通过dsRNA注射的subolesin基因沉默抑制产卵，表明subolesin参与蜱的繁殖。最后，接种重组软蜱subolesins诱导部分保护作用，导致产卵率降低。这些初步结果鼓励进一步研究使用重组subolesins作为疫苗控制软蜱感染，无论是单独或与其他特定分子的组合。(C)2011 Elsevier B. V.保留所有权利。
Subolesin/akirin is a well characterized protective antigen highly conserved across vector species and thus potentially useful for the development of a broad-spectrum vaccine for the control of arthropod infestations including hard ticks, mosquitoes, sand flies and the poultry red mite Dermanyssus gallinae. Soft ticks could be also targeted by this vaccine if proved that the soft tick subolesin orthologs are conserved and induce protective immune responses too. However, to date no soft tick subolesin orthologs have been fully characterized nor tested as recombinant antigens in vaccination trials. The objectives of the present work were to clone and characterize the subolesin orthologs from two important vector species of soft ticks as Ornithodoros erraticus and O. moubata, to evaluate the effect of subolesin gene silencing by RNAi, and to test the protective value of the recombinant antigens in vaccination trials. The obtained results demonstrate that both soft tick subolesins are highly conserved showing more than 69% and 74% identity with those of hard ticks in their nucleotide and amino acid sequences, respectively. Additionally, we demonstrate that both soft ticks possess fully operative RNAi machinery, and that subolesin gene silencing by dsRNA injection inhibits oviposition indicating the involvement of subolesin in tick reproduction. Finally, vaccination with the recombinant soft tick subolesins induced a partial protective effect resulting in the reduction of the oviposition rate. These preliminary results encourage further studies on the use of recombinant subolesins as vaccines for the control of soft tick infestations, either alone or in combination with other specific molecules. (C) 2011 Elsevier B.V. All rights reserved.