Changes in brain glycogen after sleep deprivation vary with genotype
Changes in brain glycogen after sleep deprivation vary with genotype
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DOI：
10.1152/ajpregu.00668.2002
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发表时间：
2003-08-01
影响因子：
2.8
通讯作者：
Heller, HC
中科院分区：
文献类型：
--
作者：
Franken, P;Gip, P;Heller, HC
睡眠在功能上与大脑能量稳态有关，因为它可以补充清醒时耗尽的大脑能量储存。因此，睡眠剥夺（SD）会降低脑糖原含量。我们通过剥夺三种近交系小鼠的睡眠来验证这一假设，AKR/J（AK）、DBA/2 J（D2）和C57 BL/6 J（B6），它们在睡眠调节方面差异很大。SD 6 h后，用微波照射杀死这些小鼠及其对照组，并在大脑皮质、脑干和小脑中定量糖原和葡萄糖。SD后，B6小鼠皮质中的两种测量值均显著增加约40%，而AK和D2小鼠脑干和小脑中的糖原显著减少20 - 38%。相比之下，SD后，AK小鼠所有三种结构中的葡萄糖含量增加，而D2小鼠没有变化。B6小鼠SD后糖原的增加在食物剥夺的条件下持续存在，其本身降低了皮质糖原。此外，在对睡眠丧失的补偿反应方面差异最大的菌株，即，AK和D2的糖原反应没有差异。因此，糖原含量本身不太可能是睡眠在大脑能量平衡中的功能性作用的终点。
Sleep has been functionally implicated in brain energy homeostasis in that it could serve to replenish brain energy stores that become depleted while awake. Sleep deprivation (SD) should therefore lower brain glycogen content. We tested this hypothesis by sleep depriving mice of three inbred strains, i.e., AKR/J (AK), DBA/2J (D2), and C57BL/6J (B6), that differ greatly in their sleep regulation. After a 6-h SD, these mice and their controls were killed by microwave irradiation, and glycogen and glucose were quantified in the cerebral cortex, brain stem, and cerebellum. After SD, both measures significantly increased by similar to40% in the cortex of B6 mice, while glycogen significantly decreased by 20 - 38% in brain stem and cerebellum of AK and D2 mice. In contrast, after SD, glucose content increased in all three structures in AK mice and did not change in D2 mice. The increase in glycogen after SD in B6 mice persisted under conditions of food deprivation that, by itself, lowered cortical glycogen. Furthermore, the strains that differ most in their compensatory response to sleep loss, i.e., AK and D2, did not differ in their glycogen response. Thus glycogen content per se is an unlikely end point of sleep's functional role in brain energy homeostasis.