PARASITE LACTATE-DEHYDROGENASE AS AN ASSAY FOR PLASMODIUM-FALCIPARUM DRUG-SENSITIVITY
PARASITE LACTATE-DEHYDROGENASE AS AN ASSAY FOR PLASMODIUM-FALCIPARUM DRUG-SENSITIVITY
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DOI：
10.4269/ajtmh.1993.48.739
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发表时间：
1993-06-01
影响因子：
3.3
通讯作者：
HINRICHS, DJ
中科院分区：
文献类型：
--
作者：
MAKLER, MT;RIES, JM;HINRICHS, DJ
本报告比较了使用乳酸脱氢酶（pLDH）测定与3h -次黄嘌呤结合和吉姆萨显微镜来评估抗疟疾药物对体外恶性疟原虫生长的抑制作用。pLDH法的抑制谱和IC50测定与放射性摄取法和显微法测定的结果直接相当。此外，pLDH培养敏感性试验具有可重复性、易于解释、快速和廉价的特点，表明了其在现场的适用性。
This report compares the use of the lactate dehydrogenase (pLDH) assay with 3H-hypoxanthine incorporation and Giemsa microscopy for the evaluation of anti-malaria drug inhibition of the growth of P. falciparum in vitro. The inhibition profiles and IC50 determinations of the pLDH assay were directly comparable to those determined by the radioactive uptake and microscopic methods. Furthermore, the pLDH culture sensitivity assay is reproducible, easily interpreted, rapid and inexpensive to perform, suggesting field applicability.