Development of a sandwich ELISA for evaluating soluble PD-L1 (CD274) in human sera of different ages as well as supernatants of PD-L1+ cell lines
Development of a sandwich ELISA for evaluating soluble PD-L1 (CD274) in human sera of different ages as well as supernatants of PD-L1+ cell lines
复制标题
开发夹心 ELISA，用于评估不同年龄的人血清以及 PD-L1 细胞系上清液中的可溶性 PD-L1 (CD274)。
DOI：
10.1016/j.cyto.2011.06.004
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发表时间：
2011-11-01
期刊：
影响因子：
3.8
通讯作者：
Zhang, Xueguang
中科院分区：
文献类型：
--
作者：
Chen, Yongjing;Wang, Qin;Zhang, Xueguang
PD-L1（CD 274）是一种关键的膜结合共刺激分子，可通过其受体PD-1抑制免疫反应。先前的数据表明，这种分子与自身免疫性疾病、慢性病毒感染和肿瘤免疫逃逸有关。然而，可溶性形式的人PD-L1（sPD-L1）的存在和作用仍然未知。我们在这里显示，一种新的酶联免疫吸附试验（ELISA）的开发，以检测sPD-L1蛋白。PD-L1（+）细胞系的许多培养上清液含有高水平的该因子。有趣的是，sPD-L1在人血清中是可检测的，并且浓度以年龄依赖性方式增加。人5 PD-L1在血清中具有独特的蛋白形式，基质金属蛋白酶抑制剂（MMPI）可抑制sPD-L1的产生。此外，sPD-L1可与PD-1特异性结合。总之，这些数据表明，人血清中循环sPD-L1的存在可能在免疫调节中发挥重要作用。(C)2011爱思唯尔有限公司版权所有。
PD-L1 (CD274) is a critical membrane-bound costimulatory molecule that inhibits immune responses through its receptor, PD-1. Previous data have showed that this molecule is associated with autoimmune diseases, chronic viral infections and tumor immune escape. However, the existence and role of soluble form of human PD-L1 (sPD-L1) remain unknown. We show here that a novel enzyme-linked immunosorbent assay (ELISA) was developed to detect the sPD-L1 protein. Many culture supernatants of the PD-L1(+) cell lines contain high levels of this factor. Interestingly, the sPD-L1 is detectable in human serum and the concentration increases in an age-dependent manner. Human 5PD-L1 has a unique protein form in the serum and the matrix metalloproteinase inhibitor (MMPI) could suppress sPD-L1 production. Moreover, the sPD-L1 could specially bind to PD-1. Together, these data demonstrate that the existence of circulating sPD-L1 in human serum might play an important role in immunoregulation. (C) 2011 Elsevier Ltd. All rights reserved.