Chemokine profile of herniated intervertebral discs infiltrated with monocytes and macrophages
Chemokine profile of herniated intervertebral discs infiltrated with monocytes and macrophages
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DOI：
10.1097/00007632-200207150-00006
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发表时间：
2002-07-15
期刊：
影响因子：
3
通讯作者：
Sato, N
中科院分区：
文献类型：
--
作者：
Kawaguchi, S;Yamashita, T;Sato, N
研究设计。应用逆转录聚合酶链式反应分析腰椎间盘突出症标本，以确定趋化因子的表达谱。目的：探讨在自发性退变过程中，炎性细胞在突出的椎间盘中重新聚集的机制。腰椎间盘突出症的自发性退变越来越多地被报道。虽然巨噬细胞被认为在这一过程中发挥了中心作用，但这些巨噬细胞是如何在突出的椎间盘中积聚的仍不清楚。提取36例腰椎间盘突出症手术标本、1例特发性脊柱侧弯和化脓性脊柱炎标本以及1例正常供者外周血单个核细胞。用针对CXC趋化因子(IL-8、MGSA-α、IP-10、MIG)、CC趋化因子(MCP-1、MCP-2、MCP-3、MCP-4、MIP-1α、MIP-3α、RANTES、STCP-1)、C趋化因子(淋巴管蛋白)和磷酸甘油醛管家基因的特异引物，对RNA进行逆转录，扩增得到的cDNA。冰冻切片，苏木精-伊红染色。除MCP-4外，其余趋化因子均由活化的外周血单核细胞表达。特发性脊柱侧弯和化脓性脊柱炎各1例，8个突出椎体标本中均检出磷酸甘油醛。检测10例腰椎间盘标本中趋化因子的表达。在13种趋化因子中，MCP-3、MCP-4、RANTES和IP-10在特发性脊柱侧凸患者的腰椎间盘中检测到，在感染或突出的腰椎间盘中检测到MCP-3、MCP-4、RANTES、IP-10、MIG和MGSA-α。组织学分析显示，感染的8个椎间盘和全部突出的椎间盘内均有炎性细胞的浸润。这一发现表明，在选定的人群中存在趋化特性，并且独特的趋化因子集合在这些突出的椎间盘组织的自发消退中发挥了作用。
Study Design. Herniated lumbar disc specimens were analyzed using reverse transcriptase-polymerase chain reaction to determine the profile of chemokine expression.Objective. To investigate the mechanism underlying the recruitment of inflammatory cells into herniated discs during the process of spontaneous regression.Summary of Background Data. Spontaneous regression of herniated intervertebral discs has been increasingly reported. Although macrophages are suggested to play a central role in this process, it remains unclear how these macrophages accumulate in the herniated discs.Methods. RNA was extracted from 36 surgical specimens of the herniated lumbar disc, a disc specimen of idiopathic scoliosis and pyogenic spondylitis, and activated peripheral blood mononuclear cells of a normal donor. The RNA was reverse transcribed, and the resultant cDNA was amplified by PCR using primer pairs specific to the CXC chemokines (IL-8, MGSA-alpha, IP-10, MIG), the CC chemokines (MCP-1, MCP-2, MCP-3, MCP-4, MIP-1alpha, MIP-3alpha, RANTES, STCP-1), the C chemokine (lymphotactin), and the glyceraldehyde phosphate housekeeping gene. Thin cryostat sections also were made from the disc specimens and stained with hematoxylin and eosin.Results. All the chemokines examined except MCP-4 were expressed by activated peripheral blood mononuclear cells. Glyceraldehyde phosphate was detected in 8 of 36 herniated discs and in 1 disc specimen each of idiopathic scoliosis and pyogenic spondylitis. Chemokine expression was examined for these 10 disc specimens. From among the 13 chemokines examined, MCP-3, MCP-4, RANTES, and IP-10 were detected in the disc from the idiopathic scoliosis and MCP-3, MCP-4, RANTES, IP-10, MIG, and MGSA-alpha were detected in the infected or herniated discs. Histologic analysis showed infiltration of inflammatory cells in the infected disc and all 8 herniated discs.Conclusions. The findings suggest that chemoattractive properties exist in a selected population of human intervertebral discs, and that unique sets of chemokines play a role in spontaneous regression of these herniated disc tissues.