Expression of bone-related genes in bone marrow MSCs after cyclic mechanical strain: implications for distraction osteogenesis.
Expression of bone-related genes in bone marrow MSCs after cyclic mechanical strain: implications for distraction osteogenesis.
复制标题
循环机械应变后骨髓 MSC 中骨相关基因的表达：对牵张成骨的影响
DOI：
10.4248/ijos.09021
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发表时间：
2009-09
影响因子：
14.9
通讯作者：
Hu J
中科院分区：
文献类型：
--
作者：
Qi MC;Zou SJ;Han LC;Zhou HX;Hu J
目的了解骨髓间充质干细胞(MSCs)对机械应变的反应及其基因表达谱，将有助于我们进一步了解牵张成骨的机制生物学。方法本研究对大鼠骨髓间充质干细胞进行单周期机械拉伸(0.5 Hz，2,000μɛ)。检测细胞增殖和碱性磷酸酶(ALP)活性。采用实时荧光定量RT-PCR法检测6种骨相关基因(Ets-1、bFGFIGF-II、转化生长因子-β、Cbfa1和碱性磷酸酶)的基因表达。机械刺激后即刻Ets-1的表达显著增加，而Cbfa1的表达随后升高。ALP的时间表达模式与Cbfa1的表达模式完全一致。结论机械应变可能是MSCs向成骨细胞分化的刺激因子，这些骨相关基因在MSCs对机械刺激的反应中可能发挥不同的作用。
AimUnderstanding the response of mesenchymal stem cells (MSCs) to mechanical strain and their consequent gene expression patterns will broaden our knowledge of the mechanobiology of distraction osteogenesis.MethodologyIn this study, a single period of cyclic mechanical stretch (0.5 Hz, 2,000 μɛ) was performed on rat bone marrow MSCs. Cellular proliferation and alkaline phosphatase (ALP) activity was examined. The mRNA expression of six bone‐related genes (Ets‐1, bFGF, IGF‐II, TGF‐β, Cbfa1 and ALP) was detected using real‐time quantitative RT‐PCR.ResultsThe results showed that mechanical strain can promote MSCs proliferation, increase ALP activity, and up‐regulate the expression of these genes. A significant increase in Ets‐1 expression was detected immediately after mechanical stimulation, but Cbfa1 expression becameelevated later. The temporal expression pattern of ALP coincided perfectly with Cbfa1.ConclusionThe results of this study suggest that mechanical strain may act as a stimulator to induce differentiation of MSCs into osteoblasts, and that these bone‐related genes may play different roles in the response of MSCs to mechanical stimulation.