Isomyosin distributions in rodent muscles: effects of altered thyroid state.
Isomyosin distributions in rodent muscles: effects of altered thyroid state.
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啮齿动物肌肉中的异肌球蛋白分布：甲状腺状态改变的影响。
DOI：
10.1152/jappl.1990.69.1.321
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Baldwin,KM
中科院分区：
文献类型：
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作者：
Fitzsimons,DP;Herrick,RE;Baldwin,KM
在这项研究中，我们研究了6-8周的甲状腺激素操作对成年雌性大鼠横纹肌异肌球蛋白表达的影响。将动物随机分配至三组之一：1）甲状腺功能正常对照组，2）甲状腺功能缺陷（丙基硫氧嘧啶治疗组）和3）甲状腺功能亢进（三碘甲状腺原氨酸治疗组）。甲状腺缺乏导致左心室和右心室的低腺苷三磷酸酶V3亚型分别显著增加20倍和49倍。相反，甲状腺功能亢进症诱导了一个温和的（3-11%），但显着增加高腺苷三磷酸酶V1亚型在两个心室。甲状腺功能缺乏的大鼠表现出显着增加缓慢肌球蛋白在比目鱼肌（8%）和红腓肠肌（24%），伴随着减少中间肌球蛋白在两块肌肉。有趣的是，虽然甲状腺功能亢进组的比目鱼肌（-19%）和红腓肠肌（-43%）的慢肌球蛋白亚型减少，但中间肌球蛋白亚型在两块肌肉中受到的影响不同，前者增加了5倍，后者减少了16%。此外，甲状腺功能亢进症增加了红色腓肠肌中的快肌球蛋白，而对白色腓肠肌中的相同亚型没有影响。总的来说，这些数据表明不同类型的横纹肌肌球蛋白基因对甲状腺激素的反应具有不同的特异性和敏感性。
In this study we examined the effects of 6-8 wk of thyroid hormone manipulation on striated muscle isomyosin expression in adult female rats. Animals were randomly assigned to one of three groups: 1) euthyroid controls, 2) thyroid deficient (propylthiouracil treated), and 3) hyperthyroid (triiodothyronine treated). Thyroid deficiency resulted in a marked increase in the low-adenosinetriphosphatase V3 isoform by 20- and 49-fold in the left and right ventricle, respectively. Conversely, hyperthyroidism induced a modest (3-11%) but significant increase in the high-adenosinetriphosphatase V1 isoform in both ventricles. The thyroid-deficient rats exhibited significant increases in slow myosin in both soleus (8%) and red gastrocnemius (24%), with concomitant reductions in intermediate myosin in both muscles. Interestingly, while the slow-myosin isoform was decreased in both the soleus (-19%) and the red gastrocnemius (-43%) of the hyperthyroid group, the intermediate-myosin isoform was affected differentially in the two muscles, with a fivefold increase in the former vs. a 16% decrease in the latter. Furthermore, hyperthyroidism increased the fast myosins in the red gastrocnemius while exerting no effect on the same isoforms in the white gastrocnemius. Collectively these data suggest both different specificity and sensitivity among the myosin genes of different striated muscle types in response to thyroid hormone.