Nonfilamentous C-albicans mutants are avirulent
Nonfilamentous C-albicans mutants are avirulent
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DOI：
10.1016/s0092-8674(00)80358-x
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发表时间：
1997-09-05
期刊：
影响因子：
64.5
通讯作者：
Fink, GR
中科院分区：
文献类型：
--
作者：
Lo, HJ;Kohler, JR;Fink, GR
白色念珠菌和酿酒酵母从酵母转变为丝状形式。在酵母中，这种开关由两种调节蛋白控制，即Ste 12 p和Phd 1 p。单突变株ste 12/ste 12或phd 1/phd 1是部分缺陷的，而sfe 12/ste 12 phd 1/phd 1双突变株在丝状生长中是完全缺陷的，并且是非侵入性的。假丝酵母中的cph 1/cph 1 efg 1/efg 1双突变体（Cph 1 p是Ste 12 p同源物，Efg 1 p是Phd 1 p同源物）也有丝状生长缺陷，不能形成菌丝或假菌丝以响应许多刺激，包括血清或巨噬细胞。这种念珠菌cph 1/cph 1 efg 1/efg 1双突变体，锁定在酵母菌的形式，是无毒的小鼠模型。
Candida albicans and Saccharomyces cerevisiae switch from a yeast to a filamentous form. In Saccharomyces, this switch is controlled by two regulatory proteins, Ste12p and Phd1p. Single-mutant strains, ste 12/ste12 or phd1/phd1, are partially defective, whereas the sfe12/ste12 phd1/phd1 double mutant is completely defective in filamentous growth and is noninvasive. The equivalent cph1/cph1 efg1/efg1 double mutant in Candida (Cph1p is the Ste12p homolog and Efg1p is the Phd1p homolog) is also defective in filamentous growth, unable to form hyphae or pseudohyphae in response to many stimuli, including serum or macrophages. This Candida cph1/cph1 efg1/efg1 double mutant, locked in the yeast form, is avirulent in a mouse model.