Antibiotics exacerbated colitis by affecting the microbiota, Treg cells and SCFAs in IL10-deficient mice
Antibiotics exacerbated colitis by affecting the microbiota, Treg cells and SCFAs in IL10-deficient mice
复制标题
抗生素通过影响 IL10 缺陷小鼠的微生物群、Treg 细胞和 SCFA 来加剧结肠炎
DOI：
10.1016/j.biopha.2019.108849
复制
发表时间：
2019-06-01
影响因子：
7.5
通讯作者：
Wang, Qijun
中科院分区：
文献类型：
--
作者：
Shen, Bo;Hu, Jiajia;Wang, Qijun
许多研究表明，抗生素治疗可以减轻 IL10 缺陷 (IL10(-/-)) 小鼠的结肠炎。然而，这些结果表明抗生素在预防而不是治疗已形成的结肠炎方面更成功。这些抗生素治疗仅试图部分改变肠道微生物群，而不是完全消除它。因此，我们用多抗生素治疗方案治疗IL10(-/-)小鼠，用于开发假无菌小鼠模型，以确定多抗生素是否减轻或加剧结肠炎。我们评估了接受抗生素治疗的IL10(-/-)小鼠与接受水对照的小鼠的结肠炎；此外，我们还研究了肠道微生物群、肠道免疫细胞比例和细胞因子分泌。令人惊讶的是，接受抗生素治疗的IL10(-/-)小鼠比接受水对照的小鼠有更严重的肠结肠炎和盲肠肿胀。此外，结肠中微生物群的丰度和短链脂肪酸（SCFA）的含量显着下降。此外，IL10(-/-)小鼠结肠中Treg细胞和Th1细胞的比例也减少。其机制可能是微生物群的减少导致维持肠道稳态所必需的Treg细胞和SCFA的比例减少。所有变化都会导致接受抗生素治疗的 IL10(-/-) 小鼠结肠炎进一步恶化。
Many studies have shown that antibiotic therapy can attenuate colitis in IL10-deficient (IL10(-/-)) mice. However, these results have indicated that antibiotics were more successful in preventing, rather than treating established colitis. Those antibiotic treatments attempted to only partially alter the intestinal microbiota and to not eliminate it completely. Therefore, we treated IL10(-/-) mice with the multiantibiotic regimen that was used to develop a pseudo-germ-free mouse model to determine whether multi-antibiotics attenuated or exacerbated colitis.We evaluated the colitis in IL10(-/-) mice receiving the antibiotic treatment versus those receiving the water control; furthermore, we investigated the gut microbiota, the intestinal immune cell proportions and the cytokine secretion. Surprisingly, the IL10(-/-) mice receiving the antibiotic treatment had more severe intestinal colitis and a swollen cecum than those receiving the water control. Moreover, the abundance of microbiota and content of short-chain fatty acids (SCFAs) in the colon were dramatically decreased. Additionally, the proportions of Treg cells and Th1 cells in the colons of IL10(-/-) mice were also decreased. The mechanism may be that the decrease in the microbiota leads to a decrease in the proportions of Treg cells and SCFAs, which are necessary to maintain intestinal homeostasis. All changes lead to further exacerbated colitis in IL10(-/-) mice with antibiotic treatment.