COLE1 MULTIMER FORMATION TRIGGERS INHIBITION OF ESCHERICHIA-COLI CELL-DIVISION
COLE1 MULTIMER FORMATION TRIGGERS INHIBITION OF ESCHERICHIA-COLI CELL-DIVISION
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DOI：
10.1111/j.1365-2958.1993.tb01238.x
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发表时间：
1993-09-01
影响因子：
3.6
通讯作者：
SUMMERS, DK
中科院分区：
文献类型：
--
作者：
PATIENT, ME;SUMMERS, DK
多聚体的形成和随之而来的拷贝数下降是多拷贝质粒不稳定性的公认原因。多聚体解析位点（其中ColE1 cer是最好的特点）已被确定在各种质粒。它们参与多聚体向单体的转化，使独立分离分子的数量最大化，并使质粒丢失的频率最小化。我们表明，多聚体分辨率单独是不够的，以确保稳定的维护ColE1样质粒在重组熟练的主机。还需要Rcd的表达，Rcd是在cer内编码并在含有多聚体的细胞中表达的转录物。Rcd的出现与含多聚体细胞分裂的抑制相关，推测为通过位点特异性重组将多聚体转化为单体提供了时间。
Multimer formation and consequent copy number depression are acknowledged causes of multicopy plasmid instability. Multimer resolution sites (among which ColE1 cer is best-characterized) have been identified in a variety of plasmids. They participate in the conversion of multimers to monomers, maximizing the number of independently segregating molecules and minimizing the frequency of plasmid loss. We show that multimer resolution alone is insufficient to ensure stable maintenance of ColE1-like plasmids in a recombination-proficient host. The expression of Rcd, a transcript encoded within cer and expressed in multimer-containing cells, is also required. The appearance of Rcd correlates with the inhibition of division of multimer-containing cells, presumably allowing time for the conversion of multimers to monomers by site-specific recombination.