Isolation and structure of pompilidotoxins, novel peptide neurotoxins in solitary wasp venoms
Isolation and structure of pompilidotoxins, novel peptide neurotoxins in solitary wasp venoms
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DOI：
10.1006/bbrc.1998.9299
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发表时间：
1998-09-29
影响因子：
3.1
通讯作者：
Takayama, H
中科院分区：
文献类型：
--
作者：
Konno, K;Hisada, M;Takayama, H
新的多肽神经毒素--α-和β-PMTX(α-和β-PMTX)是从独居黄蜂Anoplius samariensis和Batozonellus maculifrons的毒液中分离纯化出来的。它们的结构主要通过MALDI-TOF-MS进行了分析，并通过固相合成得到了证实。含有13个氨基酸残基和Arg-Ile-Lys-Ile-Gly-Leu-Phe-Gln-Asp-Leu-Ser-Lys-Leu-NH(2)，序列的α-PMTX通过突触前机制极大地增强了龙虾腿肌的突触传递。用精氨酸取代α-PMTX第12位赖氨酸残基的β-PMTX比α-PMTX更有效。(C)1998年学术出版社。
Novel peptide neurotoxins, alpha- and beta-pompilidotoxins (alpha- and beta-PMTXs), were purified from the venoms of the solitary wasps Anoplius samariensis and Batozonellus maculifrons. Their structures were analyzed mostly by MALDI-TOF-MS, which were corroborated by solid-phase synthesis. alpha-PMTX, with 13 amino acid residues and the sequence of Arg-Ile-Lys-Ile-Gly-Leu-Phe-Gln-Asp-Leu-Ser-Lys-Leu-NH(2), greatly potentiates synaptic transmission of lobster leg muscle by the presynaptic mechanisms. beta-PMTX, in which the lysine residue at 12 position of alpha-PMTX was replaced with arginine, was more potent than alpha-PMTX. (C) 1998 Academic Press.