Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection.
Pathogenicity of Treponema denticola Wild-Type and Mutant Strain Tested by an Active Mode of Periodontal Infection Using Microinjection.
复制标题
使用显微注射通过牙周感染的活动模式测试齿垢密螺旋体野生型和突变株的致病性。
DOI：
10.1155/2012/549169
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发表时间：
2012
影响因子：
2.1
通讯作者：
Kent,Ralph
中科院分区：
文献类型：
--
作者：
Izard,Jacques;Sasaki,Hajime;Kent,Ralph
现有的小鼠牙周感染的被动模式需要高效率的细菌附着和侵袭力，并且并不总是适合于测试基因工程突变菌株的致病性。我们开发了一种主动的口腔感染模式，在小鼠的边缘牙龈中使用显微注射，以测试与野生型菌株相比，缺乏中间样细丝的基因工程密螺旋体denticolamutant菌株的致病性。这种靶向感染模式将待测试的细菌菌株直接接种在位于未来牙周病变部位的病变部位（针进入点）。T.与假感染相比，denticola野生型菌株引起骨丢失的能力与中间体样细丝缺陷突变菌株缺乏致病性形成对比。小鼠牙周显微注射口腔模型可用于多种应用，以补充牙周感染的被动模式，在致病性测试的背景下。
The available passive mode of periodontal infections in mice requires high efficiency of bacterial attachment and invasiveness and is not always suitable to test the pathogenicity of genetically engineered mutant strains. We developed an active mode of oral infection, using microinjection in the marginal gingiva of mice, to test the pathogenicity of a genetically engineeredTreponema denticolamutant strain deficient in intermediate‐like filaments, compared to the wild‐type strain. This targeted mode of infection inoculates the bacterial strain to be tested directly at a lesion site (needle entry point) located at the future periodontal lesion site. The efficiency ofT. denticolawild‐type strain to elicit bone loss contrasted with the lack of pathogenicity of the intermediate‐like filament deficient mutant strain in comparison to the sham infection. The periodontal microinjection oral model in mice can be used for a variety of applications complementary to the passive mode of periodontal infection in context of pathogenicity testing.