Uptake and retention of sex steroids by the baboon pituitary gland--evidence of sexual dimorphism with respect to dihydrotestosterone.
Uptake and retention of sex steroids by the baboon pituitary gland--evidence of sexual dimorphism with respect to dihydrotestosterone.
复制标题
狒狒垂体对性类固醇的吸收和保留——二氢睾酮性别二态性的证据。
DOI：
10.1095/biolreprod28.2.377
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发表时间：
1983
影响因子：
3.6
通讯作者：
Sheridan,PJ
中科院分区：
文献类型：
--
作者：
Herbert,DC;Sheridan,PJ
应用放射自显影和放射自显影结合免疫细胞化学方法，对雌、雄狒狒脑垂体内雌二醇（E2）和双氢睾酮（DHT）靶细胞进行了定位和鉴定。雌、雄鼠的远侧部都有最多的3 H-E2细胞。免疫细胞化学显示，这些靶细胞主要是促性腺激素细胞和促乳腺激素细胞，虽然一些生长激素细胞和促甲状腺激素细胞也被标记。一个明显的性别二态性没有观察到相对于氚化雌激素的吸收和保留，但在这些动物given 3 H-DHT明显。显着更多的细胞在所有三个叶的雄性狒狒，远侧部，中间和神经，集中放射性标记的雄激素相比，在女性。然而，无论性别如何，促性腺激素细胞是标记的远侧部中的主要细胞类型。一小部分促乳腺生长细胞、促生长细胞和促甲状腺细胞也保留了~ 3 H-DHT。在雄性和雌性中，神经部是垂体对3 H-DHT最敏感的部分。这些研究结果表明，所有三个叶的垂体的男性和女性狒狒含有E2和DHT的靶细胞和DHT摄取的倾向是男性比女性更大。
The localization and identification of the target cells for estradiol (E2) and dihydrotestosterone (DHT) were studied in the pituitary gland of male and female baboons (Papio cynocephalus) using autoradiography and autoradiography combined with immunocytochemistry. The largest number of cells which sequestered3H-E2were located in the pars distalis of both the males and females. Immunocytochemistry revealed that these target cells were predominantly gonadotrophs and mammotrophs, albeit a few somatotrophs and thyrotrophs were also labeled. A distinct sexual dimorphism was not observed with respect to the uptake and retention of the tritiated estrogen but was clearly evident in those animals given3H-DHT. Significantly more cells in all three lobes of the male baboon, the partes distalis, intermedia and nervosa, concentrated the radiolabeled androgen as compared to that found in the females. Regardless of sex, however, the gonadotrophs were the major cell type in the pars distalis which was labeled. A small population of mammotrophs, somatotrophs and thyrotrophs also retained3H-DHT. In both males and females, the pars nervosa was the portion of the hypophysis most sensitive to3H-DHT. These findings indicate that all three lobes of the hypophysis of the male and female baboon contain target cells for E2and DHT and that the propensity for DHT uptake is greater in males than in females.