Identification of specific in vivo-induced (ivi) genes in Yersinia ruckeri and analysis of ruckerbactin, a catecholate siderophore iron acquisition system
Identification of specific in vivo-induced (ivi) genes in Yersinia ruckeri and analysis of ruckerbactin, a catecholate siderophore iron acquisition system
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DOI：
10.1128/aem.70.9.5199-5207.2004
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发表时间：
2004-09-01
影响因子：
4.4
通讯作者：
Guijarro, JA
中科院分区：
文献类型：
--
作者：
Fernández, L;Márquez, I;Guijarro, JA
这项工作报告了利用体内表达技术系统，以确定在体内诱导（ivi）的基因在鲁氏耶尔森氏菌在鱼类中选择所需的条件后。选择14个克隆，并在部分测序后分析克隆的DNA片段。除了序列没有显着的相似性，同源性与蛋白质pupillin参与的双组分和IV型分泌系统，粘附，特定的代谢功能，和others.Among这些序列，四个参与铁收购通过儿茶酚铁载体（ruckerbactin）的基因被发现。因此，与其他产生耶尔森菌素的致病耶尔森菌不同，Y. Ruckeri可能只能生产和利用这种酚盐。的ruckerbactin生物合成和摄取位点的遗传组织是类似的大肠杆菌肠杆菌素基因簇。rucC和rupG基因是E. coli entC和fepG分别参与铁载体复合物的生物合成和转运。因此，铁和温度对表达的调节以及它们在其他Y. Ruckeri产铁载体菌株证实了这两个基因座。此外，50%的致死剂量值比野生型菌株高100倍，获得与rucC同基因突变体，显示ruckerbactin的重要性，这种微生物引起的发病机制。
This work reports the utilization of an in vivo expression technology system to identify in vivo-induced (ivi) genes in Yersinia ruckeri after determination of the conditions needed for its selection in fish. Fourteen clones were selected, and the cloned DNA fragments were analyzed after partial sequencing. In addition to sequences with no significant similarity, homology with genes encoding proteins putatively involved in two-component and type IV secretion systems, adherence, specific metabolic functions, and others were found. Among these sequences, four were involved in iron acquisition through a catechol siderophore (ruckerbactin). Thus, unlike other pathogenic yersiniae producing yersiniabactin, Y. ruckeri might be able to produce and utilize only this phenolate. The genetic organization of the ruckerbactin biosynthetic and uptake loci was similar to that of the Escherichia coli enterobactin gene cluster. Genes rucC and rupG, putative counterparts of E. coli entC and fepG, respectively, involved in the biosynthesis and transport of the iron siderophore complex, respectively, were analyzed further. Thus, regulation of expression by iron and temperature and their presence in other Y. ruckeri siderophore-producing strains were confirmed for these two loci. Moreover, 50% lethal dose values 100-fold higher than those of the wild-type strain were obtained with the rucC isogenic mutant, showing the importance of ruckerbactin in the pathogenesis caused by this microorganism.