Bone mineral measurements of subchondral and trabecular bone in healthy and osteoporotic rabbits
Bone mineral measurements of subchondral and trabecular bone in healthy and osteoporotic rabbits
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DOI：
10.1007/s00256-005-0022-z
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发表时间：
2006-01-01
影响因子：
2.1
通讯作者：
Herrero-Beaumont, G
中科院分区：
文献类型：
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作者：
Castañeda, S;Largo, R;Herrero-Beaumont, G
简介：兔骨质疏松症的实验模型可用于研究合成代谢剂，因为这种动物在建模过程中具有快速的骨转换和主要的重塑。为此，有必要对每种骨组织的密度值进行表征。目的：确定健康和骨质疏松家兔脊柱和膝关节骨小梁、皮质骨和软骨下骨的面积骨量测量值。设计图：采用Hologic QDR-1000/W双能X线骨密度仪对29只成年雌性健康新西兰兔的腰椎、全膝关节、膝关节软骨下骨和皮质骨的骨矿物质含量和骨密度进行了测量。10只兔子进行了三次扫描，以评价复位的效果。15只家兔通过双侧卵巢切除术和术后皮质类固醇治疗4周，实验性诱导骨质疏松症。此后进行了相同的双能X线吸收测定法（DXA）研究。结果如下：腰椎、全膝关节、软骨下骨和皮质胫骨干骺端的骨矿物质含量平均值分别为：1934 +/- 217 mg、878 +/- 83 mg、149 +/- 14 mg和29 +/- 7.0 mg。相同部位的骨密度平均值分别为：298 +/- 24 mg/cm（2）、455 +/- 32 mg/cm（2）、617 +/- 60 mg/cm（2）和678 +/- 163 mg/cm（2）。健康家兔的骨矿含量和骨密度在所研究的四个骨骼区域遵循正态分布。三次重复定位后的精密度产生的变异系数范围为2.6%-3.8%。最小显著变化范围为7.3%至10.7%。在四个不同的骨骼区域测量的骨矿物质密度显著相关。研究的四个区域的骨质疏松兔的骨密度显着低于对照组，导致T评分分别为：腰椎-2.0 +/- 1.1，膝关节-2.2 +/- 2.1，软骨下骨-1.9 +/- 0.6，胫骨皮质骨-5.7 +/- 3.1（P < 0.05）。结论：DXA是一种可靠、准确的评价骨密度的方法。我们的研究结果还表明，软骨下骨是一种混合密度特征的骨，具有明显的皮质骨优势。
Introduction: Experimental models of osteoporosis in rabbits are useful to investigate anabolic agents because this animal has a fast bone turnover with predominant remodelling over the modelling processes. For that purpose, it is necessary to characterize the densitometric values of each type of bony tissue. Objective: To determine areal bone mass measurement in the spine and in trabecular, cortical and subchondral bone of the knee in healthy and osteoporotic rabbits. Design: Bone mineral content and bone mineral density were measured in lumbar spine, global knee, and subchondral and cortical bone of the knee with dual energy X-ray absorptiometry using a Hologic QDR-1000/W densitometer in 29 skeletally mature female healthy New Zealand rabbits. Ten rabbits underwent triplicate scans for evaluation of the effect of repositioning. Osteoporosis was experimentally induced in 15 rabbits by bilateral ovariectomy and postoperative corticosteroid treatment for 4 weeks. Identical dual energy X-ray absorptiometry (DXA) studies were performed thereafter. Results: Mean values of bone mineral content at the lumbar spine, global knee, subchondral bone and cortical tibial metaphysis were: 1934 +/- 217 mg, 878 +/- 83 mg, 149 +/- 14 mg and 29 +/- 7.0 mg, respectively. The mean values of bone mineral density at the same regions were: 298 +/- 24 mg/cm(2), 455 +/- 32 mg/cm(2), 617 +/- 60 mg/cm(2) and 678 +/- 163 mg/cm(2), respectively. Bone mineral content and bone density of healthy rabbits followed a normal distribution at the four skeletal regions studied. Precision after triplicate repositioning yielded a coefficient of variation ranging from 2.6% to 3.8%. The least significant change ranged between 7.3% and 10.7%. Bone mineral density measured at the four different skeletal regions correlated significantly. Bone mineral density in osteoporotic rabbits was significantly lower in the four regions studied than that in controls, rendering a T-score of, respectively, -2.0 +/- 1.1 in the lumbar spine, -2.2 +/- 2.1 in the global knee, -1.9 +/- 0.6 in the subchondral bone, and -5.7 +/- 3.1 in the cortical tibia (P < 0.05). Conclusions: DXA is a reliable and precise method to evaluate the bone mass in rabbits. Our results also suggest that subchondral bone is a bone of mixed densitometric characteristics with marked cortical bone predominance.