Drosophila big brain does not act as a water channel, but mediates cell adhesion
Drosophila big brain does not act as a water channel, but mediates cell adhesion
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DOI：
10.1016/j.febslet.2009.05.035
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发表时间：
2009-06
期刊：
影响因子：
3.5
通讯作者：
Kimiko Tatsumi;S. Tsuji;H. Miwa;Toshinori Morisaku;M. Nuriya;M. Orihara;K. Kaneko;H. Okano;
中科院分区：
文献类型：
--
作者：
Kimiko Tatsumi;S. Tsuji;H. Miwa;Toshinori Morisaku;M. Nuriya;M. Orihara;K. Kaneko;H. Okano;
神经原基因Drosophilabig brain（bib）与水通道蛋白-4具有高度的序列同源性。然而，其在果蝇神经发生中的细胞功能仍然是难以捉摸的。在这里，我们研究了细胞粘附，以及Bib的离子和水渗透性。通过使用L细胞的细胞聚集测定来检查粘附功能。Bib转染的L细胞形成聚集簇，而对照L细胞保持为单细胞悬液。在稳定表达Bib的L细胞中未证实离子渗透。当在COS 7细胞中表达时，Bib表现出有限的透水性。这一新发现的细胞粘附功能可能对果蝇神经发生具有重要意义。
The neurogenic geneDrosophilabig brain(bib) has a high sequence homology to aquaporin‐4. However, its cellular functions inDrosophilaneurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay using L cells. Bib‐transfected L cells formed aggregated clusters, while control‐L cells remained as a single cell suspension. Ion permeation was not confirmed in L cells stably expressing Bib. When expressed in COS7 cells, Bib exhibited limited water permeability. This newly found cell adhesive function of Bib may be important forDrosophilaneurogenesis.