Induction and Quantification of NETosis.
Induction and Quantification of NETosis.
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DOI：
10.1002/cpim.16
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发表时间：
2016-11-01
影响因子：
--
通讯作者：
Kaplan, Mariana J
中科院分区：
文献类型：
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作者：
Carmona-Rivera, Carmelo;Kaplan, Mariana J
中性粒细胞胞外陷阱（NET）是由核材料和中性粒细胞颗粒蛋白组成的网状结构，在特定微生物或无菌炎症刺激后释放到细胞外空间。 NETosis 是中性粒细胞挤出 NET 时发生的一种特定类型的细胞死亡。制定经过验证的标准来诱导和量化 NET 非常重要，以便准确比较实验室之间的结果并增进对这一生物过程及其对健康和疾病的影响的理解。该单元介绍了从外周血中分离人类中性粒细胞并通过各种类型的刺激诱导 NET 的方法，这些刺激依赖于 NADPH 氧化酶或线粒体产生活性氧。还描述了用于 NET 的低通量和高通量定量的替代方法。 © 2016 John Wiley & Sons, Inc. 版权所有
Neutrophil extracellular traps (NET) are web-like structures composed of nuclear material and neutrophil granular proteins that are released to the extracellular space after specific microbial or sterile inflammatory stimuli. NETosis is a specific type of cell death that ensues when neutrophils extrude NETs. It is important to develop validated standards to induce and quantify NETs in order to accurately compare results between laboratories and advance the understanding of this biological process and its implications in health and disease. This unit presents methods for isolating human neutrophils from peripheral blood and inducing NETs through various types of stimulation that rely on the production of reactive oxygen species from NADPH oxidase or mitochondria. Alternative methods are also described for low- and high-throughput quantification of NETs. © 2016 by John Wiley & Sons, Inc.