Intestinal absorption and biological effects of orally administered amorphous silica particles.
Intestinal absorption and biological effects of orally administered amorphous silica particles.
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DOI：
10.1186/1556-276x-9-532
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发表时间：
2014
影响因子：
--
通讯作者：
Tsutsumi Y
中科院分区：
文献类型：
--
作者：
Yoshida T;Yoshioka Y;Takahashi H;Misato K;Mori T;Hirai T;Nagano K;Abe Y;Mukai Y;Kamada H;Tsunoda S;Nabeshi H;Yoshikawa T;Higashisaka K;Tsutsumi Y
尽管无定形二氧化硅纳米颗粒广泛用于食品的生产（例如，作为防结块剂），关于口服后的吸收和生物效应的信息很少。在此，我们研究了经口给予直径为70、300和1，000 nm的无定形二氧化硅颗粒（分别为nSP 70、mSP 300和mSP 1000）以及表面经羧基或胺基修饰的nSP 70（分别为nSP 70-C和nSP 70-N）的小鼠体外肠道吸收和体内生物学效应。肠吸收的外翻肠囊法结合电感耦合等离子体发射光谱仪的分析表明，肠吸收的nSP 70-C是显着大于nSP 70。nSP 70-N的吸收倾向于大于nSP 70;然而，结果无统计学显著性。我们的研究结果表明，二氧化硅纳米粒子可以通过肠道吸收，粒径和表面性质是吸收程度的主要决定因素。我们还研究了二氧化硅颗粒在小鼠口服暴露28天后的生物学效应。血液病理学、组织病理学和生物化学分析显示，对照小鼠和用二氧化硅颗粒处理的小鼠之间没有显著差异，表明本研究中评价的二氧化硅纳米颗粒可安全用于食品生产。
Although amorphous silica nanoparticles are widely used in the production of food products (e.g., as anticaking agents), there is little information available about their absorption and biological effects after oral exposure. Here, we examined the in vitro intestinal absorption and in vivo biological effects in mice of orally administered amorphous silica particles with diameters of 70, 300, and 1,000 nm (nSP70, mSP300, and mSP1000, respectively) and of nSP70 that had been surface-modified with carboxyl or amine groups (nSP70-C and nSP70-N, respectively). Analysis of intestinal absorption by means of the everted gut sac method combined with an inductively coupled plasma optical emission spectrometer showed that the intestinal absorption of nSP70-C was significantly greater than that of nSP70. The absorption of nSP70-N tended to be greater than that of nSP70; however, the results were not statistically significant. Our results indicate that silica nanoparticles can be absorbed through the intestine and that particle diameter and surface properties are major determinants of the degree of absorption. We also examined the biological effects of the silica particles after 28-day oral exposure in mice. Hematological, histopathological, and biochemical analyses showed no significant differences between control mice and mice treated with the silica particles, suggesting that the silica nanoparticles evaluated in this study are safe for use in food production.