Three-dimensional structure of unstained, frozen-hydrated extended tails of bacteriophage T4.
Three-dimensional structure of unstained, frozen-hydrated extended tails of bacteriophage T4.
复制标题
未染色、冷冻水合的噬菌体 T4 延长尾的三维结构。
DOI：
10.1016/0022-2836(85)90202-5
复制
发表时间：
1985
影响因子：
5.6
通讯作者：
K. Leonard
中科院分区：
文献类型：
--
作者：
J. Lepault;K. Leonard
无支持的，未染色的冷冻水合延长尾部的噬菌体T4已研究了冷冻电子显微镜。在对不同粒子的信息进行相关和平均后，重建了它们的三维结构。虽然水化尾部的重建显示了传统电子显微镜发现的所有特征，但它们的特征在于开放结构。构成轴向重复序列的单个亚基不能明确地勾勒出来，因为密度连接性对相衬传递函数效应敏感。为了最大限度地减少这些影响，我们发现，最好的数据集的三维重建是由层线相衬传递函数和未校正的赤道校正。
Unsupported, unstained frozen-hydrated extended tails of bacteriophage T4 have been studied by cryo-electron microscopy. Their three-dimensional structure has been reconstructed after correlation and averaging of the information from different particles. While the reconstructions of hydrated tails show all the features found by conventional electron microscopy, they are characterized by an open structure. Individual subunits constituting the axial repeat cannot be outlined unambiguously, as the density connectivity is sensitive to the phase-contrast transfer function effects. In order to minimize these effects, we found that the best data set for three-dimensional reconstruction is composed of layer-lines corrected for the phase-contrast transfer function and an uncorrected equator.