Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins
Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins
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DOI：
10.1016/s1097-2765(00)80420-7
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发表时间：
2000-02-01
期刊：
影响因子：
16
通讯作者：
Nasmyth, K
中科院分区：
文献类型：
--
作者：
Ciosk, R;Shirayama, M;Nasmyth, K
姐妹染色单体之间的内聚依赖于多亚单位内聚蛋白复合物，该复合物在DNA复制周围与染色体结合，并在后期开始时与染色体分离。Scc2p虽然不是内聚亚基，但也是姐妹染色单体内聚所必需的。我们在这里展示了Scc2p与一种新的蛋白质Scc4p形成复合体，这也是姐妹内聚所必需的。在scc2或scc4突变体中，内聚蛋白复合物正常形成，但不能与着丝粒和染色体臂结合。我们的数据表明，Scc2p/Scc4p复合体的主要作用是促进内聚蛋白复合体装载到染色体上。
Cohesion between sister chromatids depends on a multisubunit cohesin complex that binds to chromosomes around DNA replication and dissociates from them at the onset of anaphase. Scc2p, though not a cohesin subunit, is also required for sister chromatid cohesion. We show here that Scc2p forms a complex with a novel protein, Scc4p, which is also necessary for sister cohesion. In scc2 or scc4 mutants, cohesin complexes form normally but fail to bind both to centromeres and to chromosome arms. Our data suggest that a major role for the Scc2p/Scc4p complex is to facilitate the loading of cohesin complexes onto chromosomes.