Molecular cloning and characterization of triterpene synthases from Medicago truncatula and Lotus japonicus
Molecular cloning and characterization of triterpene synthases from Medicago truncatula and Lotus japonicus
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DOI：
10.1023/a:1022519709298
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发表时间：
2003-03-01
影响因子：
5.1
通讯作者：
Osbourn, AE
中科院分区：
文献类型：
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作者：
Iturbe-Ormaetxe, I;Haralampidis, K;Osbourn, AE
从适于分子遗传学的豆类物种中克隆三萜合成所需的OSC将提供工具来解决三萜及其衍生物在正常植物生长和发育期间以及在与共生体和病原体的相互作用中的重要性。在这里，我们报告了从豆科植物蒺藜苜蓿和百脉根中克隆和鉴定了三种三萜类糖苷酶。这些包括来自M. truncatula（MtAMY 1）和来自百脉根的混合功能三萜合酶（LjAMY 2）。从L. - 是的MtAMY 1、LiAMY 1和LjAMY 2以及先前从M.蒺藜和豌豆在不同的植物组织中以及在萌发过程中存在差异，这表明这些酶可能在植物生理和发育中具有不同的作用。
Cloning of OSCs required for triterpene synthesis from legume species that are amenable to molecular genetics will provide tools to address the importance of triterpenes and their derivatives during normal plant growth and development and also in interactions with symbionts and pathogens. Here we report the cloning and characterization of a total of three triterpene synthases from the legume species Medicago truncatula and Lotus japonicus. These include a beta-amyrin synthase from M. truncatula (MtAMY1) and a mixed function triterpene synthase from Lotus japonicus (LjAMY2). A partial cDNA predicted to encode a beta-amyrin synthase (LjAMY1) was also isolated from L. japonicus. The expression patterns of MtAMY1, LiAMY1 and LjAMY2 and of additional triterpene synthases previously characterised from M. truncatula and pea differ in different plant tissues and during nodulation, suggesting that these enzymes may have distinct roles in plant physiology and development.