Inhibitory effect of high-dosage zinc oxide dietary supplementation on Campylobacter coli excretion in weaned piglets
Inhibitory effect of high-dosage zinc oxide dietary supplementation on Campylobacter coli excretion in weaned piglets
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DOI：
10.1111/jam.12307
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发表时间：
2013-11-01
影响因子：
4
通讯作者：
Alter, T.
中科院分区：
文献类型：
--
作者：
Bratz, K.;Goelz, G.;Alter, T.
AimsThis study investigated the impact of zinc oxide（ZnO）on Campylobacter coli by in vivo and in vitro assessment.Methods and ResultsBy in vitro growth inhibition assessments，a high-sensitivity of Camp.coli against ZnO can be observed.当ZnO浓度为26 mmoll（-1）时，细胞数量减少。定量实时PCR分析表明，上调的主要氧化应激基因（katA）在ZnO治疗。ZnO处理后katA的表达水平增加了5倍。在猪中进行实验以阐明ZnO作为饲料添加剂对大肠杆菌粪便排泄的影响。与低剂量（40 mgkg（-1））或中等剂量（100 mgkg（-1））ZnO饲料相比，高剂量ZnO饲料（3100 mgkg（-1））可显著降低仔猪粪便中大肠杆菌的排泄量，最高可达1 logCFU g（-1）。在断奶仔猪日粮中添加高水平的ZnO显著降低了大肠杆菌的排泄量。有证据表明，氧化锌补充剂诱导氧化应激反应在Camp.coli.Significance和影响的StudySupplementation的高剂量氧化锌饮食仔猪可以减少Camp.coli负荷，潜在地导致在屠宰过程中降低肉的污染风险。
AimsThis study investigated the impact of zinc oxide (ZnO) on Campylobacter coli by in vivo and in vitro assays.Methods and ResultsBy in vitro growth inhibition assays, a high susceptibility of Camp.coli against ZnO could be observed. At concentrations 26mmoll(-1) ZnO, a decline in cell numbers occurred. Quantitative real-time PCR assays demonstrated an up-regulation of the main oxidative stress gene (katA) in response to ZnO treatment. The expression level of katA was increased by fivefold after ZnO treatment. An experiment was carried out in pigs to elucidate the impact of ZnO as feed supplement on Camp.coli faecal excretion. Feeding a high-dosage ZnO concentration (3100mgkg(-1)) to piglets significantly reduced the faecal excretion of Camp.coli by up to 1logCFUg(-1) as compared to animals receiving a low (40mgkg(-1)) or medium (100mgkg(-1)) ZnO diet.ConclusionIn vitro assays showed a high susceptibility of Camp.coli against ZnO. Adding high levels of ZnO to the diet of weaned piglets reduced Camp.coli excretion significantly. There is evidence for the induction of an oxidative stress response by ZnO supplementation in Camp.coli.Significance and Impact of the StudySupplementation of a high-dosage ZnO diet to piglets can reduce the Camp.coli load, potentially leading to a lower contamination risk of meat during slaughter.