Loss of the CD5(+) and CD45RA(hi) B cell subsets in alcoholics
Loss of the CD5(+) and CD45RA(hi) B cell subsets in alcoholics
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DOI：
10.1046/j.1365-2249.1996.d01-621.x
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发表时间：
1996-02-01
影响因子：
4.6
通讯作者：
McLatchie, K
中科院分区：
文献类型：
--
作者：
Cook, RT;Waldschmidt, TJ;McLatchie, K
慢性酒精中毒者经常免疫缺陷，有多克隆高丙种球蛋白血症，并经常有自身抗体。最近在其他疾病中的研究表明，在由表面标志物CD 5和CD 45 RA的差异表达定义的B细胞亚群中可以发现与自身免疫和免疫缺陷可能相关的功能差异。因此，我们评估了CD 5，CD 45 RA B细胞亚群的慢性酗酒者没有证据的活动性肝病（AWLD），和酗酒者承认急性酒精性肝病（ALD）。平均B细胞数在AWLD中正常，但在ALD中显著减少。通过三色流式细胞术分析20名患者和29名对照者的B细胞，发现酗酒者的B细胞中CD 5（+）细胞的百分比急剧下降，37.6%对16.3%，P < 0.00001;绝对CD 5（+）B细胞数量也同样减少（58.9细胞/mu 1对20.9; P = 0.0012）。除了CD 5 + B细胞的丢失外，CDS（-）CD 45 RA（hi）的B细胞百分比降低，使许多患者的B细胞谱主要为CD 19（+）CD 5（-）CD 45 RA（lo）。该亚群在表型上与其他人描述的产生IgM的CD 5（-）CD 45 RA（lo）亚群相似，并且可以富集自身抗体产生细胞。一个异常值患者是ALD，其中61%的B细胞是CD 5（+），这也是与增加的自身抗体产生一致的特征。
Chronic alcoholics are frequently immunodeficient, have polyclonal hypergammaglobulinaemia, and often have autoantibodies. Recent work in other diseases has shown that functional distinctions of possible relevance to autoimmunity and immunodeficiency can be found among the B cell subsets defined by differential expression of the surface markers CD5 and CD45RA. Therefore, we have evaluated the CD5,CD45RA B cell subsets of both chronic alcoholics without evidence of active liver disease (AWLD), and alcoholics admitted for acute alcoholic liver disease (ALD). Mean B cell numbers were normal in AWLD, but significantly reduced in ALD. Analysis of B cells by three-colour flow cytometry in 20 patients and 29 controls revealed a sharp decrease in the percentage of alcoholics' B cells which were CD5(+), 37.6% versus 16.3%, P < 0.00001; absolute CD5(+) B cell numbers were similarly reduced (58.9 cells/mu 1 versus 20.9; P = 0.0012). In addition to the loss of CD5 + B cells, there was a reduction in the percentage of B cells which are CDS(-)CD45RA(hi) leaving many patients with a B cell profile which was predominantly CD19(+)CD5(-)CD45RA(lo). This subset appears phenotypically similar to the IgM-producing CD5(-)CD45RA(lo) subset described by others, and may be enriched for auto antibody-producing cells. One outlier patient was an ALD with 61% of B cells which were CD5(+), which also is a profile consistent with increased autoantibody production.