ROLE OF CAPSID PRECURSOR PROCESSING AND MYRISTOYLATION IN MORPHOGENESIS AND INFECTIVITY OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1
ROLE OF CAPSID PRECURSOR PROCESSING AND MYRISTOYLATION IN MORPHOGENESIS AND INFECTIVITY OF HUMAN IMMUNODEFICIENCY VIRUS TYPE-1
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DOI：
10.1073/pnas.86.15.5781
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
HASELTINE, WA
中科院分区：
文献类型：
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作者：
GOTTLINGER, HG;SODROSKI, JG;HASELTINE, WA
描述了影响衣壳前体蛋白翻译后加工的突变对人类免疫缺陷病毒1型病毒粒子形态发生和病毒复制的影响。从N末端的位置2处的甘氨酸残基的变化废除了前体蛋白的肉豆蔻酰化，并且还防止了病毒颗粒释放。病毒蛋白酶基因的突变消除了衣壳前体的蛋白水解裂解，但不能阻止未成熟外观的病毒粒子颗粒的形成和出芽。改变通常用于从衣壳前体切割主要衣壳蛋白p24的位点序列的突变改变病毒体形态发生并阻止病毒复制。
The effect on human immunodeficiency virus type 1 virion morphogenesis and on virus replication of mutations that affect posttranslational processing of the capsid precursor protein are described. A change in the glycine residue at position two from the N terminus abolished the myristoylation of the precursor proteins and also prevents virus particle release. Mutations in the viral protease gene abolish proteolytic cleavage of the capsid precursor but do not prevent the formation and budding of virion particles of immature appearance. Mutations that alter the sequence of the sites normally used for cleavage of the major capsid protein p24 from the capsid precursor alter virion morphogenesis and prevent virus replication.