The comparative study of Sprague-Dawley and Lewis rats in adjuvant-induced arthritis
The comparative study of Sprague-Dawley and Lewis rats in adjuvant-induced arthritis
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DOI：
10.1007/s00210-006-0062-5
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发表时间：
2006-05-01
影响因子：
3.6
通讯作者：
Liu, L.
中科院分区：
文献类型：
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作者：
Cai, X.;Wong, Y. F.;Liu, L.
近交系Spraogue-Dawley(SD)大鼠与近交系Lewis(LEW)大鼠相似，最近被证明对佐剂性关节炎(AIA)高度易感。在此，我们从临床、组织学、放射学和免疫炎症特征方面比较了SD大鼠和LEW大鼠的AIA。结果表明，接种结核分枝杆菌悬液后，SD大鼠和LEW大鼠的病情发展相似，发病率均为100%，病情严重程度相似。伴随着关节炎的发展，大鼠的血沉(ESR)和C反应蛋白(CRP)水平显著高于对照组。后爪X线检查显示，SD和LEW AIA大鼠均表现出明显的软组织肿胀、骨基质吸收、骨膜新骨形成和骨侵蚀，而滑膜关节的组织病理学检查显示明显的细胞浸润、血管生成、滑膜增生、血管疙瘩形成、关节间隙变窄以及软骨和骨破坏。此外，在疾病进展过程中，SD和LEW AIA大鼠血清肿瘤坏死因子-α(TNF-α)、白介素1-β(IL-1β)和IL-6水平均显著高于对照组，且SD和LEW AIA大鼠血清中肿瘤坏死因子-α(TNF-α)和IL-1β的表达特征不同。综上所述，这些结果表明，SD大鼠AIA模型与LEW大鼠的可比模型具有一些共同的关节炎特征。因此，考虑到SD大鼠比LOW大鼠更有利的特点(即成本低、可获得性广、背景异质性)，这种SD大鼠AIA模型更具成本效益，更有利于筛选和测试新型抗关节炎药物。
The outbred Sprague-Dawley (SD) rats, similar to the inbred Lewis (LEW) rats, have been recently demonstrated to be highly susceptible to adjuvant-induced arthritis (AIA). We herein compared AIA in SD and LEW rats in terms of clinical, histological, radiological, and immuno-inflammatory features. The results showed that, following inoculation with a ground Mycobacterium tuberculosis (MT) suspension, SD and LEW rats manifested closely similar disease progression, with 100% incidence and similar severity. The development of arthritis was accompanied by significantly higher erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) levels than in control rats. Radiographic examination of the hind paws showed that both SD and LEW AIA rats manifested conspicuous soft tissue swelling, bone matrix resorption, periosteal new bone formation and bone erosion, while histopathological analysis of the synovial joints revealed marked cellular infiltration, angiogenesis, synovial hyperplasia, pannus formation, narrowing of joint space, and cartilage and bone destruction. Moreover, in relation to disease progression, serum tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and IL-6 levels were markedly overexpressed in both SD and LEW AIA versus control rats, and SD and LEW AIA rats exhibited divergent profiles for the expression of TNF-alpha and IL-1 beta. Taken together, these results demonstrated that the SD rat AIA model shares several arthritic features with the comparable model in LEW rats. Hence, given the more favorable characteristics of SD rats than LEW rats (i.e., lower cost, wider availability, and heterogenic background), this SD rat AIA model is more cost effective and advantageous for screening and testing novel anti-arthritic agents.