Neuronal specific regulatory elements in apolipoprotein E gene proximal promoter
Neuronal specific regulatory elements in apolipoprotein E gene proximal promoter
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DOI：
10.1097/00001756-200506210-00029
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发表时间：
2005-06-21
期刊：
影响因子：
1.7
通讯作者：
Bullido, MJ
中科院分区：
文献类型：
--
作者：
Ramos, MC;Matías, S;Bullido, MJ
载脂蛋白E （ApoE）数量的全身和局部变化与阿尔茨海默病和其他神经退行性疾病有关，显示维持生理ApoE水平的相关性。然而，APOE转录在神经细胞中的研究并不广泛。在本报告中，我们研究了不同APOE近端启动子区域的转录活性及其与人类神经（星形细胞瘤和神经母细胞瘤）和外周（肝癌和淋巴瘤）细胞系核蛋白的结合。我们在所有细胞中定义了几个具有负调控作用的区域，在神经母细胞瘤细胞中定义了一个具有强阳性活性的区域。此外，我们发现-219T/G多态性会产生APOE转录活性的变化，其中G等位基因表现出更高的活性。(c) 2005年Lippincott Williams & Wilkins。
Systemic and local changes in apolipoprotein E (ApoE) quantity have been related with Alzheimer's and other neurodegenerative diseases, showing the relevance of maintaining physiological ApoE levels. However, APOE transcription has not been extensively studied in neural cells. In this report, we study the transcriptional activity of different APOE proximal promoter regions and their binding to nuclear proteins from human neural (astrocytoma and neuroblastoma) and peripheral (hepatoma and lymphoma) cell lines. We define several regions with a negative regulatory effect in all the cells and a region with a strong positive activity in neuroblastoma cells. Additionally, we show that the -219T/G polymorphism produces variations in APOE transcriptional activity, with the G allele showing higher activity. (c) 2005 Lippincott Williams & Wilkins.