Curcumin promotes cholesterol efflux from adipocytes related to PPARgammaLXRalphaABCA1 passway
Curcumin promotes cholesterol efflux from adipocytes related to PPARgammaLXRalphaABCA1 passway
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DOI：
10.1007/s11010-011-0978-z
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发表时间：
2011-12-01
影响因子：
4.3
通讯作者：
Nie, Sai
中科院分区：
文献类型：
--
作者：
Dong, Shao-zhuang;Zhao, Shui-ping;Nie, Sai
姜黄素影响脂肪细胞的功能。但姜黄素是否对脂肪细胞的胆固醇流出过程有一定的影响尚不清楚。将兔皮下脂肪细胞与5、10和20 μ g/ml姜黄素孵育24小时。评估胆固醇流出到apoAI上，并通过逆转录聚合酶链反应（RT-PCR）定量脂肪细胞中过氧化物酶体增殖物激活受体（PPAR）γ、肝脏X受体（LXR）α和ATP结合盒转运蛋白A1（ABCA 1）mRNA的表达。姜黄素以剂量依赖方式增加脂肪细胞胆固醇流出。姜黄素引起的PPAR γ、LXR α和ABCA 1表达的增加是平行的。当脂肪细胞用GW 9662预处理时，姜黄素诱导的PPAR γ表达增加被部分阻止，随后LXR α和ABCA 1下调。姜黄素可通过调节PPAR γ-LXR-ABCA 1通路影响脂肪细胞胆固醇流出。
Curcumin affects the functions of adipocytes. But it is not known whether curcumin has some effect on the cholesterol efflux process of adipocytes. Rabbit subcutaneous adipocytes were incubated with 5, 10 and 20 mu g/ml curcumin for 24 h. The cholesterol efflux onto apoAI was assessed, and the peroxisome proliferators-activated receptor (PPAR) gamma, liver X receptor (LXR) a and ATP-binding cassette transporter A1 (ABCA1) mRNA expression in adipocytes were quantified by reverse-transcription polymerase chain reaction (RT-PCR). Curcumin increased the cholesterol efflux from adipocytes in dose-dependent manner. The increased expression of PPAR gamma, LXR alpha and ABCA1 caused by curcumin were parallel. When the adipocytes were pre-treated by GW9662, the increased expression of PPAR gamma induced by curcumin was partially prevented, subsequent to the down- regulation of LXRa and ABCA1. Curcumin can affect the cholesterol efflux from adipocytes by regulating the PPAR gamma-LXR-ABCA1 passway.