Metabolic profiling using combined GC-MS and LC-MS provides a systems understanding of aristolochic acid-induced nephrotoxicity in rat
Metabolic profiling using combined GC-MS and LC-MS provides a systems understanding of aristolochic acid-induced nephrotoxicity in rat
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DOI：
10.1016/j.febslet.2007.01.036
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发表时间：
2007-02-20
期刊：
影响因子：
3.5
通讯作者：
Jia, Wei
中科院分区：
文献类型：
--
作者：
Ni, Yan;Su, Mingming;Jia, Wei
我们在这里提出了一种联合GC-MS和LC-MS代谢分析方法来揭示马兜铃酸（AA）诱导的肾毒性的病理结果。采用气相色谱-质谱和液相色谱-质谱结合主成分分析（PCA）、正交投影到潜在结构判别分析等模式识别技术对尿样进行分析。本研究表明，组织病理学检查表明，aa引起的急性肾毒性可能以全身代谢网络紊乱为特征，包括游离脂肪酸生成、能量和氨基酸代谢以及肠道微生物群结构的改变。因此，该方法可能适用于毒理学研究，提供对系统对外源干预反应的全面理解。(c) 2007年欧洲生化学会联合会。Elsevier B.V.版权所有。
We present here a combined GC-MS and LC-MS metabolic profiling approach to unraveling the pathological outcomes of aristolochic acid (AA)-induced nephrotoxicity. Urine samples were analyzed by GC-MS and LC-MS in combination with pattern recognition techniques, e.g. principal component analysis (PCA), orthogonal projection to latent structure-discriminant analysis. The work indicates that AA-induced acute renal toxicity as evidenced by histopathological examinations could be characterized by systemic disturbance of metabolic network involving free fatty acids generation, energy and amino acids metabolism, and alteration in the structure of gut microbiota. Therefore, this method is potentially applicable to the toxicological study, providing a comprehensive understanding of systems response to xenobiotic intervention. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.