Coupling of transient near infrared photonic with magnetic nanoparticle for potential dissipation-free biomedical application in brain.
Coupling of transient near infrared photonic with magnetic nanoparticle for potential dissipation-free biomedical application in brain.
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DOI：
10.1038/srep29792
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发表时间：
2016-07-28
影响因子：
4.6
通讯作者：
Nair M
中科院分区：
文献类型：
--
作者：
Sagar V;Atluri VSR;Tomitaka A;Shah P;Nagasetti A;Pilakka-Kanthikeel S;El-Hage N;McGoron A;Takemura Y;Nair M
基于磁性纳米粒子(MNPs)和近红外线(NIR)生物光子学的联合治疗策略在非侵入性治疗方法方面具有巨大的潜力。尽管如此，这方面的研究一直局限于外周躯体区域，对这种方法在脑内的潜在生物医学应用知之甚少。在此，我们报道，在存在或不存在超顺磁性纳米颗粒的情况下，瞬时近红外辐射是无耗散的，并且对主要脑细胞的活性和可塑性没有不利影响。采用带有热电偶的808 nm近红外激光组件对脑细胞进行近红外照射的功能研究。利用透射电子显微镜、X射线衍射仪、动态激光散射仪和振动样品磁强计对磁性纳米颗粒进行了表征。用细胞-底物阻抗传感系统、细胞毒性评价和共聚焦显微镜分析脑细胞的活性和可塑性。当有效的非侵入性光生物调节和神经治疗对脑的靶向和监测仍然是一项艰巨的任务时，这种无耗散的、瞬时的脑细胞近红外光子方法的发现具有显著的潜力增加新的维度。
Combined treatment strategies based on magnetic nanoparticles (MNPs) with near infrared ray (NIR) biophotonic possess tremendous potential for non-invasive therapeutic approach. Nonetheless, investigations in this direction have been limited to peripheral body region and little is known about the potential biomedical application of this approach for brain. Here we report that transient NIR exposure is dissipation-free and has no adverse effect on the viability and plasticity of major brain cells in the presence or absence superparamagnetic nanoparticles. The 808 nm NIR laser module with thermocouple was employed for functional studies upon NIR exposure to brain cells. Magnetic nanoparticles were characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic laser scattering (DLS), and vibrating sample magnetometer (VSM). Brain cells viability and plasticity were analyzed using electric cell-substrate impedance sensing system, cytotoxicity evaluation, and confocal microscopy. When efficacious non-invasive photobiomodulation and neuro-therapeutical targeting and monitoring to brain remain a formidable task, the discovery of this dissipation-free, transient NIR photonic approach for brain cells possesses remarkable potential to add new dimension.