Protein expression is increased by a class III AU-rich element and tethered CUG-BP1
Protein expression is increased by a class III AU-rich element and tethered CUG-BP1
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DOI：
10.1016/j.bbrc.2006.06.177
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发表时间：
2006-09-01
影响因子：
3.1
通讯作者：
Paillard, Luc
中科院分区：
文献类型：
--
作者：
Barreau, Carine;Watrin, Tanguy;Paillard, Luc
在哺乳动物体细胞中，细胞因子或原一基因表达的转录后控制通常通过与3'非翻译区（3' UTR）中的序列元件结合的因子来实现。研究最多的是富Au元素（ARE），它被分为三类。在这里，我们表明，在哺乳动物细胞中，在报告mRNA的3 'UTR中存在III类c-jun ARE增强了报告蛋白的表达。相反，在3 'UTR中存在II类ARE降低了报告蛋白的表达。CUG-BP 1/CELF 1能够结合c-jun ARE。当报告基因mRNA含有合成的CUG-BP 1/CELF 1结合位点时，或当该蛋白质与报告基因mRNA的3 'UTR连接时，蛋白质表达与c-jun ARE观察到的类似地增强。这些结果揭示了ARE介导的转录后调控的意想不到的复杂性，并表明CUG-BP 1/CELF 1在III类ARE定向调控中的功能。(c)2006年爱思唯尔公司All rights reserved.
In mammalian somatic cells, the post-transcriptional control of cytokine or proto-oneogene expression is often achieved by factors binding to sequence elements in the 3' untranslated region (3'UTR). The most studied are the AU-rich elements (ARE) that have been divided into three classes. Here, we show that in mammalian cells, the presence of the class III c-jun ARE in the 3'UTR of a reporter mRNA enhanced reporter protein expression. In contrast, the presence of a class II ARE in the 3'UTR decreased reporter protein expression. CUG-BP1/CELF1 is able to bind c-jun ARE. Protein expression was enhanced similarly to what was observed for c-jun ARE when the reporter mRNA contained a synthetic CUG-BP1/CELF1-binding site, or when this protein was tethered to the 3'UTR of a reporter mRNA. These results reveal an unexpected complexity of ARE-mediated post-transcriptional regulations, and indicate a function for CUG-BP1/CELF1 in class III ARE directed regulations., (c) 2006 Elsevier Inc. All rights reserved.