Dysregulated synthesis of intracellular type 1 and type 2 cytokines by T cells of patients with cutaneous T-cell lymphoma
Dysregulated synthesis of intracellular type 1 and type 2 cytokines by T cells of patients with cutaneous T-cell lymphoma
复制标题
DOI：
10.1128/cdli.6.1.79-84.1999
复制
发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
Reuben, JM
中科院分区：
文献类型：
--
作者：
Lee, BN;Duvic, M;Reuben, JM
蕈样肉芽肿（MF）和Sezary综合征（SS）是皮肤T细胞淋巴瘤（CTCL）的两个主要临床实体。随着疾病从MF进展到SS，发生从1型（白细胞介素[IL]-2和γ干扰素[IFN-γ]）到2型（IL-4）细胞因子产生谱的转变。虽然1型和2型细胞因子在CTCL发病机制中的作用已被提出，但这些细胞因子的细胞起源尚不清楚。使用流式细胞术，以确定个人的T细胞亚群，我们研究了T细胞的细胞因子合成的13例SS和12例MF和9名血液健康的捐助者。在用佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）活化后，合成IL-2的T细胞的数目对于所有研究组是相似的。而在健康供体和MF患者中产生IL-2的主要T细胞是CD 7（+），而在SS患者中则是CD 7（-）。尽管所有研究组的IL-4（+）CD 4（+）T细胞数量均较低，但MF患者的IL-4（+）CD 8（+）T细胞数量显著高于SS患者或健康供体。与健康供体相比，CTCL供体中产生IFN-γ的T细胞数量下降。更重要的是，随着疾病从MF进展到SS，产生IFN-γ的T细胞的数量显著减少。这些T细胞不能合成IFN-γ可能在CTCL中具有预后价值，因为它可能是疾病从MF进展到SS的原因。
Mycosis fungoides (MF) and Sezary syndrome (SS) are the two main clinical entities of cutaneous T-cell lymphoma (CTCL), As the disease progresses from MF to SS, a switch from a type 1 (interleukin [IL]-2 and gamma interferon [IFN-gamma]) to a type 2 (IL-4) cytokine production profile occurs. Although roles for type 1 and type 2 cytokines in the pathogenesis of CTCL have been proposed, the cellular origins of these cytokines are unclear. Using flow cytometry to identify individual T-cell subsets, we studied cytokine synthesis by the T cells of 13 patients with SS and 12 with MF and 9 hematologically healthy donors. Upon activation with phorbol 12-myristate 13-acetate (PMA), the numbers of T cells synthesizing IL-2 were similar for all study groups. Whereas the predominant T-cell producing IL-2 in healthy donors and in those with MF was CD7(+), in patients with SS, it was CD7(-). Although the number of IL-4(+) CD4(+) T cells was low for all study groups, there was a significantly higher number of IL-4(+) CD8(+) T cells in patients with MF than in those with SS or healthy donors. There was a decline in the number of IFN-gamma-producing T cells in CTCL donors compared to that in healthy donors. More importantly, there was a significant decrease in the number of IFN-gamma-producing T cells with disease progression from MF to SS, The inability of these T cells to synthesize IFN-gamma may have prognostic value in CTCL, since it may be responsible for the progression of the disease from MF to SS.