Microbial communities associated with benthic faunal assemblages at cold seep sediments of the Sonora Margin, Guaymas Basin
Microbial communities associated with benthic faunal assemblages at cold seep sediments of the Sonora Margin, Guaymas Basin
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DOI：
10.3389/fmars.2015.00053
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发表时间：
2015-01-01
影响因子：
3.7
通讯作者：
Cambon-Bonavita, Marie-Anne
中科院分区：
文献类型：
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作者：
Cruaud, Perrine;Vigneron, Adrien;Cambon-Bonavita, Marie-Anne
索诺拉陆缘冷泉呈现出由不同的动物群和微生物席组成的海底镶嵌图案。为了更好地了解沉积微生物群落是否反映了这种斑块状分布，使用四种互补方法对所有主要栖息地进行了调查：16 S rRNA基因序列454焦磷酸测序，定量聚合酶链反应，荧光原位杂交和地球化学分析。这项研究表明，不同的表面组合填充沉积物显示出不同的孔隙水地球化学特征，并与不同的微生物群落。在沉积物的微生物垫和周围的大型底栖动物，微生物群落占主导地位的厌氧甲烷氧化菌（古菌厌氧甲烷氧化菌ANME）和硫酸盐还原Deltaproteobacteria。相比之下，与沉积物相关的微生物群落的巨型动物栖息地（vesicomyids和siboglinids）的特点是较低的生物量和重要比例的海洋底栖动物D组（MBG-D），Chloroflexi以及丝状Gammaproteobacteria和Deltaproteobacteria。总之，地球化学和微生物调查表明，孔隙水甲烷浓度在微生物群落结构中起着重要的作用，随后在建立的表面殖民者。此外，表面殖民者的存在和活动影响底层微生物群落可能是因为能源的可用性的修改。
The Sonora Margin cold seeps present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To better understand if sedimentary microbial communities reflect this patchy distribution, all major habitats were investigated using four complementary approaches: 16S rRNA gene sequence 454 pyrosequencing, quantitative polymerase chain reaction, fluorescence in situ hybridization and geochemistry analyses. This study reveals that sediments populated by different surface assemblages show distinct porewater geochemistry features and are associated with distinct microbial communities. In the sediments underlying the microbial mat and the surrounding macrofauna, microbial communities were dominated by anaerobic methane oxidizers (archaeal anaerobic methanotroph ANME) and sulfate-reducing Deltaproteobacteria. In contrast, sediment-associated microbial communities underlying the megafauna habitats (vesicomyids and siboglinids) were characterized by a lower biomass and important proportions of the Marine Benthic Group D (MBG-D), Chloroflexi as well as filamentous Gammaproteobacteria and Deltaproteobacteria. Together, geochemical and microbial surveys indicate that porewater methane concentrations play an important role in the microbial community structure and subsequently in the establishment of the surface colonizers. Furthermore, presence and activity of the surface colonizers influence the underlying microbial communities probably because of modification of energy source availabilities.